Cholesterol-3-beta, 5-alpha, 6-beta-triol induced genotoxicity through reactive oxygen species formation

Y. W. Cheng, J. J. Kang, Y. L. Shih, Y. L. Lo, C. F. Wang

研究成果: 雜誌貢獻文章

35 引文 (Scopus)

摘要

The mutagenicity of oxysterols, cholesterol-3β,5α,6β-triol (α-Triol), 7-keto-cholesterol (7-Keto) and cholesterol-5α,6α- epoxide (α-Epox) were examined by the Ames method and chromosome aberration test in this study. Only α-Triol concentration-dependently caused an increase of bacterial revertants in the absence of metabolic activating enzymes (S9), but not 7-keto and α-Epox. The mutagenic effect of α-Triol was reduced by the addition of S9. On the other hand, although α-Triol significantly induced chromosome aberration in CHO-K1 cells with and without S9. However, the addition of S9 reduced the degree of abnormal structure chromosome compared to without S9 mix. Catalase and superoxide dismutase (SOD) inhibited α-Triol induced increase of revertants in Salmonella typhimurium and chromosome aberration frequency in CHO cells, suggesting that reactive oxygen species (ROS) might be involved in the genotoxic effect of α-Triol. Treatment with α-Triol increased the ROS production in CHO cells, which could be attenuated by catalase and SOD. Results in this study suggested, for the first time that α-Triol, causes genotoxic effect in an ROS-dependent manner.
原文英語
頁(從 - 到)617-622
頁數6
期刊Food and Chemical Toxicology
43
發行號4
DOIs
出版狀態已發佈 - 四月 2005

指紋

chromosome aberrations
CHO Cells
genotoxicity
Chromosomes
Chromosome Aberrations
reactive oxygen species
Reactive Oxygen Species
Aberrations
cholesterol
Catalase
Superoxide Dismutase
catalase
superoxide dismutase
Cholesterol
Chromosome Structures
mutagenicity
cells
Salmonella typhimurium
epoxides
Salmonella Typhimurium

ASJC Scopus subject areas

  • Food Science
  • Toxicology

引用此文

Cholesterol-3-beta, 5-alpha, 6-beta-triol induced genotoxicity through reactive oxygen species formation. / Cheng, Y. W.; Kang, J. J.; Shih, Y. L.; Lo, Y. L.; Wang, C. F.

於: Food and Chemical Toxicology, 卷 43, 編號 4, 04.2005, p. 617-622.

研究成果: 雜誌貢獻文章

Cheng, Y. W. ; Kang, J. J. ; Shih, Y. L. ; Lo, Y. L. ; Wang, C. F. / Cholesterol-3-beta, 5-alpha, 6-beta-triol induced genotoxicity through reactive oxygen species formation. 於: Food and Chemical Toxicology. 2005 ; 卷 43, 編號 4. 頁 617-622.
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abstract = "The mutagenicity of oxysterols, cholesterol-3β,5α,6β-triol (α-Triol), 7-keto-cholesterol (7-Keto) and cholesterol-5α,6α- epoxide (α-Epox) were examined by the Ames method and chromosome aberration test in this study. Only α-Triol concentration-dependently caused an increase of bacterial revertants in the absence of metabolic activating enzymes (S9), but not 7-keto and α-Epox. The mutagenic effect of α-Triol was reduced by the addition of S9. On the other hand, although α-Triol significantly induced chromosome aberration in CHO-K1 cells with and without S9. However, the addition of S9 reduced the degree of abnormal structure chromosome compared to without S9 mix. Catalase and superoxide dismutase (SOD) inhibited α-Triol induced increase of revertants in Salmonella typhimurium and chromosome aberration frequency in CHO cells, suggesting that reactive oxygen species (ROS) might be involved in the genotoxic effect of α-Triol. Treatment with α-Triol increased the ROS production in CHO cells, which could be attenuated by catalase and SOD. Results in this study suggested, for the first time that α-Triol, causes genotoxic effect in an ROS-dependent manner.",
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AU - Lo, Y. L.

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AB - The mutagenicity of oxysterols, cholesterol-3β,5α,6β-triol (α-Triol), 7-keto-cholesterol (7-Keto) and cholesterol-5α,6α- epoxide (α-Epox) were examined by the Ames method and chromosome aberration test in this study. Only α-Triol concentration-dependently caused an increase of bacterial revertants in the absence of metabolic activating enzymes (S9), but not 7-keto and α-Epox. The mutagenic effect of α-Triol was reduced by the addition of S9. On the other hand, although α-Triol significantly induced chromosome aberration in CHO-K1 cells with and without S9. However, the addition of S9 reduced the degree of abnormal structure chromosome compared to without S9 mix. Catalase and superoxide dismutase (SOD) inhibited α-Triol induced increase of revertants in Salmonella typhimurium and chromosome aberration frequency in CHO cells, suggesting that reactive oxygen species (ROS) might be involved in the genotoxic effect of α-Triol. Treatment with α-Triol increased the ROS production in CHO cells, which could be attenuated by catalase and SOD. Results in this study suggested, for the first time that α-Triol, causes genotoxic effect in an ROS-dependent manner.

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