Chitosan inhibits the rehabilitation of damaged microbes induced by photodynamic inactivation

Ching Hsuan Lin, Hsiung Fei Chien, Ming Hsuan Lin, Chueh Pin Chen, Mandy Shen, Chin Tin Chen

研究成果: 雜誌貢獻文章

2 引文 (Scopus)

摘要

Previously, we showed that chitosan could augment the biocidal efficacy mediated by photodynamic treatment against Staphylococcus aureus, Pseudomonas aeruginosa, and Candida albicans. In this study, we showed that the antimicrobial action of chitosan in augmenting photodynamic inactivation (PDI) is related to the increase in cell surface destruction. The microbial cell surfaces exhibit severe irregular shapes after PDI in the presence of chitosan as demonstrated by transmitted electron microscopy. Furthermore, increases in the concentration or incubation time of chitosan significantly reduced the amounts of photosensitizer toluidine blue O required, indicating that chitosan could be an augmenting agent used in conjunction with PDI against S. aureus, P. aeruginosa, and C. albicans. A prolonged lag phase was found in microbial cells that survived to PDI, in which chitosan acted to completely eradicate the cells. Once the exponential log stage and cell rebuild began, their cellular functions from PDI-induced damage returned and the increased cytotoxic effect of chitosan disappeared. Together, our results suggest that chitosan can prevent the rehabilitation of PDI-surviving microbial cells, leading to increased biocidal efficacy.
原文英語
文章編號2598
期刊International Journal of Molecular Sciences
19
發行號9
DOIs
出版狀態已發佈 - 九月 1 2018

指紋

Chitosan
microorganisms
Patient rehabilitation
deactivation
Rehabilitation
cells
Candida albicans
Pseudomonas aeruginosa
Staphylococcus aureus
pseudomonas
staphylococcus
Microbial Viability
Tolonium Chloride
destruction
Photosensitizing Agents
Photosensitizers
Candida
electron microscopy
time lag
Electron microscopy

ASJC Scopus subject areas

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

引用此文

Chitosan inhibits the rehabilitation of damaged microbes induced by photodynamic inactivation. / Lin, Ching Hsuan; Chien, Hsiung Fei; Lin, Ming Hsuan; Chen, Chueh Pin; Shen, Mandy; Chen, Chin Tin.

於: International Journal of Molecular Sciences, 卷 19, 編號 9, 2598, 01.09.2018.

研究成果: 雜誌貢獻文章

Lin, Ching Hsuan ; Chien, Hsiung Fei ; Lin, Ming Hsuan ; Chen, Chueh Pin ; Shen, Mandy ; Chen, Chin Tin. / Chitosan inhibits the rehabilitation of damaged microbes induced by photodynamic inactivation. 於: International Journal of Molecular Sciences. 2018 ; 卷 19, 編號 9.
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AU - Chen, Chin Tin

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