Chitinase activity of sweet potato (Ipomoea batatas [L.] Lam var. Tainong 57)

Wen C. Hou, Ying Chou Chen, Yaw Huei Lin

研究成果: 雜誌貢獻文章

19 引文 (Scopus)

摘要

Chitinase activity was detected in the crude extracts of different parts of sweet potato (Ipomoea batatas [L.] Lam var. Tainong 57) using commercial deacetyl glycol chitin as a substrate. The activity of chitinase in crude extracts, which was expressed as units/g fresh weight, was arranged in decending order of leaves > sprouts > peels of storage roots > peeled storage roots. Under field conditions, the chitinase activity of leaves was higher than vines. The chitinase activity of insect-bitten leaves was higher than that of the controls, and the chitinase activity of vines attached to insect-bitten leaves was also higher than that of vines attached to intact leaves. The molecular weight of main chitinase determined by activity staining was about 16 kDa. The optimum pH was 5.0 and the optimum temperature of chitinase activity was 25°C.

原文英語
頁(從 - 到)93-97
頁數5
期刊Botanical Bulletin of Academia Sinica
39
發行號2
出版狀態已發佈 - 1998
對外發佈Yes

指紋

Ipomoea batatas
sweet potatoes
chitinase
vines
leaves
glycols
insects
extracts
chitin
molecular weight
temperature

ASJC Scopus subject areas

  • Plant Science

引用此文

Chitinase activity of sweet potato (Ipomoea batatas [L.] Lam var. Tainong 57). / Hou, Wen C.; Chen, Ying Chou; Lin, Yaw Huei.

於: Botanical Bulletin of Academia Sinica, 卷 39, 編號 2, 1998, p. 93-97.

研究成果: 雜誌貢獻文章

@article{63dea2f5a948455e9ebfb5e887055c1d,
title = "Chitinase activity of sweet potato (Ipomoea batatas [L.] Lam var. Tainong 57)",
abstract = "Chitinase activity was detected in the crude extracts of different parts of sweet potato (Ipomoea batatas [L.] Lam var. Tainong 57) using commercial deacetyl glycol chitin as a substrate. The activity of chitinase in crude extracts, which was expressed as units/g fresh weight, was arranged in decending order of leaves > sprouts > peels of storage roots > peeled storage roots. Under field conditions, the chitinase activity of leaves was higher than vines. The chitinase activity of insect-bitten leaves was higher than that of the controls, and the chitinase activity of vines attached to insect-bitten leaves was also higher than that of vines attached to intact leaves. The molecular weight of main chitinase determined by activity staining was about 16 kDa. The optimum pH was 5.0 and the optimum temperature of chitinase activity was 25°C.",
keywords = "Chitinase, Defense against pests, Ipomoea batatas",
author = "Hou, {Wen C.} and Chen, {Ying Chou} and Lin, {Yaw Huei}",
year = "1998",
language = "English",
volume = "39",
pages = "93--97",
journal = "Botanical Studies",
issn = "1817-406X",
publisher = "Academia Sinica",
number = "2",

}

TY - JOUR

T1 - Chitinase activity of sweet potato (Ipomoea batatas [L.] Lam var. Tainong 57)

AU - Hou, Wen C.

AU - Chen, Ying Chou

AU - Lin, Yaw Huei

PY - 1998

Y1 - 1998

N2 - Chitinase activity was detected in the crude extracts of different parts of sweet potato (Ipomoea batatas [L.] Lam var. Tainong 57) using commercial deacetyl glycol chitin as a substrate. The activity of chitinase in crude extracts, which was expressed as units/g fresh weight, was arranged in decending order of leaves > sprouts > peels of storage roots > peeled storage roots. Under field conditions, the chitinase activity of leaves was higher than vines. The chitinase activity of insect-bitten leaves was higher than that of the controls, and the chitinase activity of vines attached to insect-bitten leaves was also higher than that of vines attached to intact leaves. The molecular weight of main chitinase determined by activity staining was about 16 kDa. The optimum pH was 5.0 and the optimum temperature of chitinase activity was 25°C.

AB - Chitinase activity was detected in the crude extracts of different parts of sweet potato (Ipomoea batatas [L.] Lam var. Tainong 57) using commercial deacetyl glycol chitin as a substrate. The activity of chitinase in crude extracts, which was expressed as units/g fresh weight, was arranged in decending order of leaves > sprouts > peels of storage roots > peeled storage roots. Under field conditions, the chitinase activity of leaves was higher than vines. The chitinase activity of insect-bitten leaves was higher than that of the controls, and the chitinase activity of vines attached to insect-bitten leaves was also higher than that of vines attached to intact leaves. The molecular weight of main chitinase determined by activity staining was about 16 kDa. The optimum pH was 5.0 and the optimum temperature of chitinase activity was 25°C.

KW - Chitinase

KW - Defense against pests

KW - Ipomoea batatas

UR - http://www.scopus.com/inward/record.url?scp=0031960704&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031960704&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:0031960704

VL - 39

SP - 93

EP - 97

JO - Botanical Studies

JF - Botanical Studies

SN - 1817-406X

IS - 2

ER -