Chicken model of steroid-induced bone marrow adipogenesis using proteome analysis: A preliminary study

Sing C. Li, Ching Y. Lin, Tzong F. Kuo, Yun H. Lin, Chia C. Chen, Way N. Lin, Wing P. Chan

研究成果: 雜誌貢獻文章

6 引文 斯高帕斯(Scopus)

摘要

Background: Steroid-induced adipogenesis increases fat-cell volume and pressure in bone marrow. This may be a contributing factor in some forms of osteonecrosis. In this observational study, we aimed to determine the protein expression relating to steroid-induced adipogenesis of femoral bone marrow with use of a chicken model. We compared the histologic features of the femoral marrow of eight methylprednisolone (MP)-treated chickens with those of three control chickens and assessed differential proteins with 2-dimensional gel electrophoresis and differential proteins were identified by MALDI-TOF MS.Results: One MP-induced chicken died of overdose anesthesia. Methylprednisolone-induced proliferation of adipose tissue and new bone formation were found on histologic examination. In our study, 13 proteins in the control and MP-induced groups were differently expressed and nine protein spots showed marked threefold downregulation after 19 weeks of MP treatment. These were serum amyloid P-component precursor, zinc finger protein 28, endothelial zinc finger protein 71, T-box transcription factor 3, cyclin-dependent kinase inhibitor 1, myosin 1D, dimethylaniline monooxygenase, and two uncharacterized proteins.Conclusions: Proteomic profiling can be a useful dynamic approach for detecting protein expression in MP-induced adipogenesis of the femur in chickens.
原文英語
文章編號47
期刊Proteome Science
8
DOIs
出版狀態已發佈 - 九月 14 2010

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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