Characterizing regulatory and functional differentiation between maize mesophyll and bundle sheath cells by transcriptomic analysis

Yao-Ming Chang, Wen-Yu Liu, Chun-Chieh Shih, Meng-Ni Shen, Chen-Hua Lu, Mei-Yeh Lu, Hui-Wen Yang, Tzi-Yuan Wang, Chun-Chang Chen, Stella Maris Chen, Wen-Hsiung Li, Mauricesb Ku

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83 引文 斯高帕斯(Scopus)

摘要

To study the regulatory and functional differentiation between the mesophyll (M) and bundle sheath (BS) cells of maize (Zea mays), we isolated large quantities of highly homogeneous M and BS cells from newly matured second leaves for transcriptome profiling by RNA sequencing. A total of 52,421 annotated genes with at least one read were found in the two transcriptomes. Defining a gene with more than one read per kilobase per million mapped reads as expressed, we identified 18,482 expressed genes; 14,972 were expressed in M cells, including 53 M-enriched transcription factor (TF) genes, whereas 17,269 were expressed in BS cells, including 214 BS-enriched TF genes. Interestingly, many TF gene families show a conspicuous BS preference in expression. Pathway analyses reveal differentiation between the two cell types in various functional categories, with the M cells playing more important roles in light reaction, protein synthesis and folding, tetrapyrrole synthesis, and RNA binding, while the BS cells specialize in transport, signaling, protein degradation and posttranslational modification, major carbon, hydrogen, and oxygen metabolism, cell division and organization, and development. Genes coding for several transporters involved in the shuttle of C4 metabolites and BS cell wall development have been identified, to our knowledge, for the first time. This comprehensive data set will be useful for studying M/BS differentiation in regulation and function. © 2012 American Society of Plant Biologists.
原文英語
頁(從 - 到)165-177
頁數13
期刊Plant Physiology
160
發行號1
DOIs
出版狀態已發佈 - 2012
對外發佈Yes

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    Chang, Y-M., Liu, W-Y., Shih, C-C., Shen, M-N., Lu, C-H., Lu, M-Y., Yang, H-W., Wang, T-Y., Chen, C-C., Chen, S. M., Li, W-H., & Ku, M. (2012). Characterizing regulatory and functional differentiation between maize mesophyll and bundle sheath cells by transcriptomic analysis. Plant Physiology, 160(1), 165-177. https://doi.org/10.1104/pp.112.203810