Characterization of the mutagen 2-amino-3-methylimidazo[4,5-f]quinoline prepared from a 2-methylpyridine/creatinine/acetylformaldehyde model system

H. Lee, M. Y. Lin, S. T. Lin

研究成果: 雜誌貢獻文章

10 引文 斯高帕斯(Scopus)

摘要

A mixture of 2-methylpyridine, creatinine and aldehydes was heated in diethylene glycol containing 5% water for 1 h at 140°C. The mutagenic compounds were purified by XAD-2 column chromatography, acid/base partition, blue cotton treatment, thin layer chromatography and HPLC. The active substances purified from each step were monitored by their mutagenicity with Salmonella typhimurium TA98 in the presence of S9 mix. Among the mutagens collected, 2-amino-3-methylimidazo[4,5-f]quinoline (IQ) was isolated from HPLC, and was identified by its UV and mass spectrum using a photodiode array detector and mass spectrometry. Our findings appear to be the first experimental evidence to substantiate the hypothetical pathway for the formation of IQ mutagens from a heated model system consisting of a pyridine or pyrazine derivative, an aldehyde and creatinine or creatine.
原文英語
頁(從 - 到)157-162
頁數6
期刊Mutagenesis
9
發行號2
出版狀態已發佈 - 1994
對外發佈Yes

ASJC Scopus subject areas

  • Genetics
  • Health, Toxicology and Mutagenesis
  • Genetics(clinical)
  • Toxicology
  • Statistics, Probability and Uncertainty
  • Applied Mathematics

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