Characterization of the first clinical isolate of vancomycin-resistant Enterococcus faecalis, AH803, in Taiwan

Jang J. Lu, Ren J. Ben, Cherng L. Perng, Wei Ming Chi, Mong Ling Chu, Wei H. Lee

研究成果: 雜誌貢獻文章

4 引文 斯高帕斯(Scopus)

摘要

We previously isolated a vancomycin-resistant strain of Enterococcus faecalis, designated AH803, from the sputum of a patient with pneumonia and bacteremia in Taiwan. AH803 was resistant to vancomycin (minimal inhibitory concentration, MIC = 512 μg/mL) but susceptible to teicoplanin (MIC = 8 μg/mL), and harbored the vanA gene but not the vanB gene. In this study, we further characterized E. faecalis AH803 and the plasmid it was found to contain. DNA from AH803 was analyzed for the presence of vanA and vanB resistance genes by polymerase chain reaction. The vancomycin resistant phenotype was transferable from AH803 to E. faecalis JH2-2, at a frequency of 4.8 x 10-2 AH803 was also resistant to gentamicin and chloramphenicol, and these antibiotic resistance phenotypes cotransferred with vancomycin resistance. The genes responsible for resistance to all three antibiotics were located on a 42-kb conjugative plasmid (pBL101). This plasmid had the same restriction enzyme digestion patterns as Tn 1546, found in piP816 of E. faecalis BM4147. Epidemiologic studies of glycopeptide resistance should perhaps combine phenotypic and genotypic methods, rather than using phenotypic methods alone.
原文英語
頁(從 - 到)178-181
頁數4
期刊Journal of the Formosan Medical Association = Taiwan yi zhi
99
發行號2
出版狀態已發佈 - 二月 2000
對外發佈Yes

ASJC Scopus subject areas

  • Medicine(all)

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