Characterization of pulmonary protein profiles in response to zinc oxide nanoparticles in mice

A 24-hour and 28-day follow-up study

Chih Hong Pan, Kai Jen Chuang, Jen Kun Chen, Ta Chih Hsiao, Ching Huang Lai, Tim P. Jones, Kelly A. BéruBé, Gui Bing Hong, Kin Fai Ho, Hsiao Chi Chuang

研究成果: 雜誌貢獻文章

3 引文 (Scopus)

摘要

Although zinc oxide nanoparticles (ZnONPs) are recognized to cause systemic disorders, little is known about the mechanisms that underlie the time-dependent differences that occur after exposure. The objective of this study was to investigate the mechanistic differences at 24 hours and 28 days after the exposure of BALB/c mice to ZnONPs via intratracheal instillation. An isobaric tag for the relative and absolute quantitation coupled with liquid chromatography/tandem mass spectrometry was used to identify the differential protein expression, biological processes, molecular functions, and pathways. A total of 18 and 14 proteins displayed significant changes in the lung tissues at 24 hours and 28 days after exposure, respectively, with the most striking changes being observed for S100-A9 protein. Metabolic processes and catalytic activity were the main biological processes and molecular functions, respectively, in the responses at the 24-hour and 28-day follow-up times. The glycolysis/gluconeogenesis pathway was continuously downregulated from 24 hours to 28 days, whereas detoxification pathways were activated at the 28-day time-point after exposure. A comprehensive understanding of the potential time-dependent effects of exposure to ZnONPs was provided, which highlights the metabolic mechanisms that may be important in the responses to ZnONP.
原文英語
頁(從 - 到)4705-4716
頁數12
期刊International Journal of Nanomedicine
10
DOIs
出版狀態已發佈 - 七月 22 2015

指紋

Zinc Oxide
Zinc oxide
Nanoparticles
Proteins
Biological Phenomena
Lung
Detoxification
Gluconeogenesis
S100 Proteins
Liquid chromatography
Glycolysis
Tandem Mass Spectrometry
Liquid Chromatography
Mass spectrometry
Catalyst activity
Down-Regulation
Tissue

ASJC Scopus subject areas

  • Biophysics
  • Bioengineering
  • Biomaterials
  • Organic Chemistry
  • Drug Discovery

引用此文

Characterization of pulmonary protein profiles in response to zinc oxide nanoparticles in mice : A 24-hour and 28-day follow-up study. / Pan, Chih Hong; Chuang, Kai Jen; Chen, Jen Kun; Hsiao, Ta Chih; Lai, Ching Huang; Jones, Tim P.; BéruBé, Kelly A.; Hong, Gui Bing; Ho, Kin Fai; Chuang, Hsiao Chi.

於: International Journal of Nanomedicine, 卷 10, 22.07.2015, p. 4705-4716.

研究成果: 雜誌貢獻文章

Pan, Chih Hong ; Chuang, Kai Jen ; Chen, Jen Kun ; Hsiao, Ta Chih ; Lai, Ching Huang ; Jones, Tim P. ; BéruBé, Kelly A. ; Hong, Gui Bing ; Ho, Kin Fai ; Chuang, Hsiao Chi. / Characterization of pulmonary protein profiles in response to zinc oxide nanoparticles in mice : A 24-hour and 28-day follow-up study. 於: International Journal of Nanomedicine. 2015 ; 卷 10. 頁 4705-4716.
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abstract = "Although zinc oxide nanoparticles (ZnONPs) are recognized to cause systemic disorders, little is known about the mechanisms that underlie the time-dependent differences that occur after exposure. The objective of this study was to investigate the mechanistic differences at 24 hours and 28 days after the exposure of BALB/c mice to ZnONPs via intratracheal instillation. An isobaric tag for the relative and absolute quantitation coupled with liquid chromatography/tandem mass spectrometry was used to identify the differential protein expression, biological processes, molecular functions, and pathways. A total of 18 and 14 proteins displayed significant changes in the lung tissues at 24 hours and 28 days after exposure, respectively, with the most striking changes being observed for S100-A9 protein. Metabolic processes and catalytic activity were the main biological processes and molecular functions, respectively, in the responses at the 24-hour and 28-day follow-up times. The glycolysis/gluconeogenesis pathway was continuously downregulated from 24 hours to 28 days, whereas detoxification pathways were activated at the 28-day time-point after exposure. A comprehensive understanding of the potential time-dependent effects of exposure to ZnONPs was provided, which highlights the metabolic mechanisms that may be important in the responses to ZnONP.",
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