Characterization of ovomucoid-specific T-cell lines and clones from egg-allergic subjects

Philippe A. Eigenmann, Shau Ku Huang, Hugh A. Sampson

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27 引文 斯高帕斯(Scopus)

摘要

In the pathogenesis of allergic reactions, T cells and cytokines play a major role. However, characterizations of food allergen-specific T cells are very limited. In this study, we screened the peripheral blood mononuclear cells (PBMC) of 14 patients for reactivity to ovomucoid (Gal d I), the major hen's egg allergen, and ovalbumin (Gal d II). Cell lines and clones specific to ovomucoid were generated from PBMC of four egg-allergic subjects, in order to study antigen domain specificity and cell cytokine production profiles. The results demonstrated, firstly, that egg-allergic patients respond to ovomucoid rather than to ovalbumin, and, secondly, that antigen specificity is predominantly directed toward the second and third domains of ovomucoid. The T-cell cytokine message was characterized by reverse transcriptase polymerase chain reaction (RT-PCR). Cell lines and clones from all four patients consistently expressed interleukin (IL)-5. IL-4, IL-13, and interferon-gamma were found to be expressed only by certain lines or clones. This observation suggests a central pathogenic role for IL-5 in food allergy-related symptoms.

原文英語
頁(從 - 到)12-21
頁數10
期刊Pediatric Allergy and Immunology
7
發行號1
DOIs
出版狀態已發佈 - 二月 1996

ASJC Scopus subject areas

  • 兒科、圍產兒和兒童健康
  • 免疫學和過敏
  • 免疫學

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