Characterization of human growth hormone from acromegalic pituitary tumors

WEN‐CHANG ‐C CHANG, ALBERT LY‐YOUNG SHEN, CHEN‐KUNG ‐K CHOU, LOW‐TONE ‐T HO

研究成果: 雜誌貢獻文章同行評審

7 引文 斯高帕斯(Scopus)

摘要

Human growth hormone (HGH) was extracted from acromegalic pituitary tumors at pH 10.5 and precipitated with ammonium sulfate at 20–40% saturation. It was purified on a Sephadex G‐100 column to yield monomeric HGH. The tumor‐HGH was indistinguishable from the authentic one in polyacrylamide gel electrophoresis at pH 8.3 or in the presence of sodium dodecyl sulfate, high‐performance liquid chromatography, radioimmunoassay, peptide map, amino acid composition and N‐terminal partial amino acid sequence. The tumor‐HGH is active in the tibia assay and body weight gain test in hypophysectomized rats with comparable potency to that of the authentic sample.
原文英語
頁(從 - 到)637-641
頁數5
期刊International Journal of Peptide and Protein Research
23
發行號6
DOIs
出版狀態已發佈 - 一月 1 1984
對外發佈Yes

ASJC Scopus subject areas

  • Biochemistry

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