Characterization of glycerophospholipids and a sphingolipid in the cerebrospinal fluid of patients with different illnesses by LC-ESI-MS

Jui Yu Wu, Ming Thau Sheu, Jia Wei Lin, Yi Bo Liou, Wen Ta Chiu, Hsiu O. Ho

研究成果: 雜誌貢獻文章

摘要

A sensitive and accurate reversed-phase liquid chromatography coupled with electrospray ionization mass spectrometry (LC-ESI-MS) method for determining glycerophospholipids and a sphingolipid was developed and validated in order to profile and quantify the classes of lipids in the cerebrospinal fluids of patients with three different illnesses. The LC-ESI-MS method was optimized for the analysis, and an Inertsil 6 ODS-3 (4.6 x 150 mm) column was utilized with a mobile phase composed of acetonitrile/methanol/triethylamine in the ratio 550/1000/25 (w/w/w) eluted isocratically at a flow rate of 1.0 mL/min. The results demonstrated that both the accuracy and precision of the intra- and inter-day assays of phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylinositol (PI), phosphatidylserine (PS) and sphingomyelin (SM) were within acceptable criteria. Clinical application demonstrated that the percentage and content of each class differed in the cerebrospinal fluids of patients with three different illnesses, with the richest lipid components being SM (848 ± 75.5 ng/mL, 40.7% ± 2.2%), PC (8042.5 ± 1214.4 ng/mL, 53.8% ± 4.2%) and PS (2124.2 ± 152.0 ng/mL, 43.3% ± 1.6%), respectively for patients with chordoma, trigeminal neuralgia and Parkinson's disease. It was concluded that this improved method can be used to directly detect glycerophospholipids and a sphingolipid, and applied to detect physiological changes in humans to discover preventive medicines or therapeutic medicines, or develop new drugs.
原文英語
期刊Journal of Food and Drug Analysis
20
發行號1
出版狀態已發佈 - 三月 2012

指紋

Glycerophospholipids
sphingolipids
Sphingolipids
Electrospray Ionization Mass Spectrometry
cerebrospinal fluid
Liquid Chromatography
liquid chromatography
Cerebrospinal Fluid
sphingomyelins
phosphatidylserines
Sphingomyelins
Phosphatidylserines
Phosphatidylcholines
phosphatidylcholines
medicine
triethylamine
Chordoma
Lipids
Trigeminal Neuralgia
Preventive Medicine

ASJC Scopus subject areas

  • Food Science
  • Pharmacology

引用此文

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title = "Characterization of glycerophospholipids and a sphingolipid in the cerebrospinal fluid of patients with different illnesses by LC-ESI-MS",
abstract = "A sensitive and accurate reversed-phase liquid chromatography coupled with electrospray ionization mass spectrometry (LC-ESI-MS) method for determining glycerophospholipids and a sphingolipid was developed and validated in order to profile and quantify the classes of lipids in the cerebrospinal fluids of patients with three different illnesses. The LC-ESI-MS method was optimized for the analysis, and an Inertsil 6 ODS-3 (4.6 x 150 mm) column was utilized with a mobile phase composed of acetonitrile/methanol/triethylamine in the ratio 550/1000/25 (w/w/w) eluted isocratically at a flow rate of 1.0 mL/min. The results demonstrated that both the accuracy and precision of the intra- and inter-day assays of phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylinositol (PI), phosphatidylserine (PS) and sphingomyelin (SM) were within acceptable criteria. Clinical application demonstrated that the percentage and content of each class differed in the cerebrospinal fluids of patients with three different illnesses, with the richest lipid components being SM (848 ± 75.5 ng/mL, 40.7{\%} ± 2.2{\%}), PC (8042.5 ± 1214.4 ng/mL, 53.8{\%} ± 4.2{\%}) and PS (2124.2 ± 152.0 ng/mL, 43.3{\%} ± 1.6{\%}), respectively for patients with chordoma, trigeminal neuralgia and Parkinson's disease. It was concluded that this improved method can be used to directly detect glycerophospholipids and a sphingolipid, and applied to detect physiological changes in humans to discover preventive medicines or therapeutic medicines, or develop new drugs.",
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AU - Sheu, Ming Thau

AU - Lin, Jia Wei

AU - Liou, Yi Bo

AU - Chiu, Wen Ta

AU - Ho, Hsiu O.

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