Characterization of collagen matrices crosslinked using microbial transglutaminase

Ray Neng Chen, Hsiu O. Ho, Ming Thau Sheu

研究成果: 雜誌貢獻文章同行評審

97 引文 斯高帕斯(Scopus)

摘要

In search of a new approach for crosslinking collagen-based biomaterials, we examined the effect of microbial transglutaminase (MTGases) as a crosslinking reagent on collagenous matrices made from porcine type I collagen. As the results revealed, MTGase exhibited a crosslinking action that raised the viscosity of the collagen solution. Matrices crosslinked with MTGase at the low pH values of pH 3 and 4 exhibited higher tensile strengths than those at high pH values. In comparison with untreated matrices, the denaturation temperatures of the corresponding matrices shifted toward higher temperatures. These enzyme-catalyzed crosslinked matrices were proven by MTT assay to be non-cytotoxic. In conclusion, this enzymatic method of using MTGase provides an alternative potential way for crosslinking collagen-based matrices.

原文英語
頁(從 - 到)4229-4235
頁數7
期刊Biomaterials
26
發行號20
DOIs
出版狀態已發佈 - 7月 2005

ASJC Scopus subject areas

  • 生物技術
  • 生物工程
  • 生物醫學工程

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