Characterization of collagen isolation and application of collagen gel as a drug carrier

Hsiu O. Ho, Lun Huei Lin, Ming Thau Sheu

研究成果: 雜誌貢獻文章同行評審

45 引文 斯高帕斯(Scopus)

摘要

The effects of various purification conditions on the characteristics of telopepeptide-poor collagen fiber were compared. It was first confirmed that a 1:50 ratio of pepsin to skin was favorable for the digestion of porcine skin. The morphological characteristics observed by scanning electron microscopy (SEM) showed that fibril collagen, porous fibril membrane or dense membrane were all possibly formed depending on the digestion and freeze-drying media. Analysis by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and size-exclusion HPLC revealed that the collagen sample produced by digestion with pepsin in a pH 2.5 HCl solution to obtain telopeptide-poor collagen, and freeze-drying in 0.5 M acetic acid for the final step, resulted in less oligomers. Not only were its fibril characteristics qualitatively identical to those of standard collagen but also were easily hydrated suggesting that this procedure is an appropriate choice for both isolation and purification. Furthermore, it was demonstrated that gel formulations prepared by gelling the vehicle mixture of citric acid solution, ethanol and propylene glycol with 1% w/w of such a collagen sample was suitable for transdermal delivery.

原文英語
頁(從 - 到)103-112
頁數10
期刊Journal of Controlled Release
44
發行號2-3
DOIs
出版狀態已發佈 - 二月 17 1997

ASJC Scopus subject areas

  • 藥學科學

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