Characterization of collagen gel solutions and collagen matrices for cell culture

Ming Thau Sheu, Ju Chun Huang, Geng Chang Yeh, Hsiu O. Ho

研究成果: 雜誌貢獻文章

179 引文 斯高帕斯(Scopus)

摘要

The influence of glutaraldehyde as a crosslinking agent to increase the strength of collagen matrices for cell culture was examined in this study. Collagen solutions of 1% were treated with different concentrations (0-0.2%) of glutaraldehyde for 24h. The viscoelasticity of the resulting collagen gel solution was measured using dynamic mechanical analysis (DMA), which demonstrated that all collagen gel solutions examined followed the same model pattern. The creep compliance model of Voigt-Kelvin satisfactorily described the change of viscoelasticity expressed by these collagen gel solutions. These crosslinked collagen gel solutions were freeze-dried to form a matrix with a thickness of about 0.2-0.3mm. The break modulus of these collagen matrices measured by DMA revealed that the higher the degree of crosslinking, the higher the break modulus. The compatibility of fibroblasts isolated from nude mouse skin with these collagen matrices was found to be acceptable at a cell density of 3×105cells/cm2 with no contraction, even when using a concentration of glutaraldehyde of up to 0.2%.

原文英語
頁(從 - 到)1713-1719
頁數7
期刊Biomaterials
22
發行號13
DOIs
出版狀態已發佈 - 2001

    指紋

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering

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