Characterization of an unique RNA initiated immediately upstream from human α1 globin gene in vivo and in vitro: Polymerase II-dependence, tissue specificity, and subcellular location

John Hess, Carlos Perez-Stable, Al Deisseroth, Che Kun James Shen

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6 引文 斯高帕斯(Scopus)

摘要

We have identified an abundant transcript initiated upstream from the canonical cap site of human α1 globin gene in bone marrow cells and in COS-7 cells transfected with an α1 globin gene-containing plasmid. Similar to the major α1 globin transcript, this upstream RNA is present almost exclusively in the cytoplasm of the transfected COS-7 cells. It is also synthesized efficiently in vitro by RNA polymerase II in the nuclear extracts prepared from a Hela cell line and an erythroleukemia cell line, K562. RNAs isolated from these cell lines, however, do not contain this upstream transcript. The putative 5′ end of the α1 globin upstream RNA is mapped by primer extension to base -45, which is located in between the CCAAT and TATA boxes. The synthesis of this RNA in vitro and in vivo, and the close proximity of its 5′ end to the promoter of the α1 globin gene suggest a common mechanism regulating the transcriptional initiation of both the upstream and the major α1 globin RNAs.

原文英語
頁(從 - 到)6059-6074
頁數16
期刊Nucleic Acids Research
13
發行號17
DOIs
出版狀態已發佈 - 九月 11 1985
對外發佈

ASJC Scopus subject areas

  • 遺傳學

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