Characterization of a novel and potent collagen antagonist, caffeic acid phenethyl ester, in human platelets: In vitro and in vivo studies

George Hsiao, Jie J. Lee, Kuang H. Lin, Chia H. Shen, Tsorng H. Fong, Duen S. Chou, Joen R. Sheu

研究成果: 雜誌貢獻文章同行評審

39 引文 斯高帕斯(Scopus)

摘要

Objective: Caffeic acid phenethyl ester (CAPE), which is derived from the propolis of honeybee hives, has been demonstrated to possess multiple pharmacological activities. In the present study, CAPE (6-25 μM) specifically inhibited collagen-induced platelet aggregation and the ATP release reaction in platelet suspensions. Methods: Platelet aggregation, flow cytometric analysis, immunoblotting, and electron spin resonance (ESR) were used to assess the anti-platelet activity of CAPE. Fluorescein sodium-induced platelet thrombi in mesenteric microvessels of mice were used for an in vivo study. Results: CAPE (15-100 μM) produced a concentration-related rightward displacement of the collagen concentration-response curve, and the Schild plot gave pA2 and pA10 values of 4.28 ± 0.07 and 3.14 ± 0.73, respectively, with a slope of - 0.83 ± 0.16, indicating specific antagonism. CAPE (25 μM) also inhibited platelet aggregation stimulated by the glycoprotein VI agonist, convulxin, and the α2β1 integrin agonist, aggretin. CAPE (25 μM) also markedly interfered with FITC-collagen binding to platelet membranes. CAPE (15 and 25 μM) concentration-dependently inhibited collagen-induced platelet activation accompanied by [Ca+2]i mobilization, phosphoinositide breakdown, activation of protein kinase C and mitogen-activated protein kinases (i.e., ERK2, JNK, and p38 MAPK), Akt phosphorylation, and thromboxane A2 formation. In the ESR study, CAPE (15 and 25 μM) markedly reduced hydroxyl radical (OH{radical dot}) formation in collagen-activated platelets. In an in vivo study, CAPE (5 mg/kg) significantly prolonged the latency in inducing platelet plug formation in mesenteric venules of mice. Conclusions: The most important findings of this study suggest that CAPE specifically inhibits collagen-induced platelet activation. Thus, CAPE treatment may represent a novel approach to lowering the risk of or improving function in thromboembolism-related disorders.
原文英語
頁(從 - 到)782-792
頁數11
期刊Cardiovascular Research
75
發行號4
DOIs
出版狀態已發佈 - 九月 1 2007

ASJC Scopus subject areas

  • 心臟病學與心血管醫學

指紋

深入研究「Characterization of a novel and potent collagen antagonist, caffeic acid phenethyl ester, in human platelets: In vitro and in vivo studies」主題。共同形成了獨特的指紋。

引用此