Characterization of a newly established malignant meningioma cell line of the human brain: IOMM-Lee

研究成果: 雜誌貢獻文章

58 引文 (Scopus)

摘要

A permanent malignant meningioma (MM) cell line of the human brain designated 'IOMM-Lee' is reported. This cell line was successfully established from the tumor of a 61-year-old Chinese man with repeated recurrent primary intraosseous malignant meningioma of the skull. It has been subcultured for more than 60 passages during the past 30 months. The doubling time of cultured cells is approximately 62 hours. Tumorigenicity in athymic nude mice (Balb/c-nu/nu) who develop multiple pulmonary metastases was observed; the doubling time of tumor volume in vivo is approximately 5 days. Karyotypic analysis revealed this cell line to be of human origin and near-diploid, with a modal chromosome number of 49. The mesenchymal tumor marker vimentin and intracytoplasmic microfilaments were identified in the cytoplasm of tumor cells by indirect immunohistochemical peroxidase-anti-peroxidase assays and immunogold ultrastructural localization by transmission electron microscopy, respectively. SCanning electron microscopy of cultured cells and xenografted tumors revealed ellipsoidal or carrot-shaped tumor cells presenting a wrinkled surface with short sparse microvilli. Potential proliferating activity was determined by Ki-67 monoclonal antibody; the Ki-67 labeling index of cultured cells and xenografted tumors was approximately 36% and 30%, respectively. This newly established malignant meningioma cell line of the human brain may prove useful as a research model.

原文英語
頁(從 - 到)389-396
頁數8
期刊Neurosurgery
27
發行號3
出版狀態已發佈 - 1990
對外發佈Yes

指紋

Meningioma
Cultured Tumor Cells
Cell Line
Brain
Nude Mice
Peroxidase
Neoplasms
Daucus carota
Vimentin
Tumor Biomarkers
Microvilli
Tumor Burden
Diploidy
Transmission Electron Microscopy
Actin Cytoskeleton
Skull
Electron Scanning Microscopy
Cultured Cells
Cytoplasm
Chromosomes

ASJC Scopus subject areas

  • Clinical Neurology
  • Surgery

引用此文

Characterization of a newly established malignant meningioma cell line of the human brain : IOMM-Lee. / Lee, W. H.

於: Neurosurgery, 卷 27, 編號 3, 1990, p. 389-396.

研究成果: 雜誌貢獻文章

@article{dd63644ed4e546d490db2d0d57ec4092,
title = "Characterization of a newly established malignant meningioma cell line of the human brain: IOMM-Lee",
abstract = "A permanent malignant meningioma (MM) cell line of the human brain designated 'IOMM-Lee' is reported. This cell line was successfully established from the tumor of a 61-year-old Chinese man with repeated recurrent primary intraosseous malignant meningioma of the skull. It has been subcultured for more than 60 passages during the past 30 months. The doubling time of cultured cells is approximately 62 hours. Tumorigenicity in athymic nude mice (Balb/c-nu/nu) who develop multiple pulmonary metastases was observed; the doubling time of tumor volume in vivo is approximately 5 days. Karyotypic analysis revealed this cell line to be of human origin and near-diploid, with a modal chromosome number of 49. The mesenchymal tumor marker vimentin and intracytoplasmic microfilaments were identified in the cytoplasm of tumor cells by indirect immunohistochemical peroxidase-anti-peroxidase assays and immunogold ultrastructural localization by transmission electron microscopy, respectively. SCanning electron microscopy of cultured cells and xenografted tumors revealed ellipsoidal or carrot-shaped tumor cells presenting a wrinkled surface with short sparse microvilli. Potential proliferating activity was determined by Ki-67 monoclonal antibody; the Ki-67 labeling index of cultured cells and xenografted tumors was approximately 36{\%} and 30{\%}, respectively. This newly established malignant meningioma cell line of the human brain may prove useful as a research model.",
keywords = "cell culture, electron microscopy, immunogold, Ki-67 labeling index, malignant meningioma, nude mice",
author = "Lee, {W. H.}",
year = "1990",
language = "English",
volume = "27",
pages = "389--396",
journal = "Neurosurgery",
issn = "0148-396X",
publisher = "Lippincott Williams and Wilkins",
number = "3",

}

TY - JOUR

T1 - Characterization of a newly established malignant meningioma cell line of the human brain

T2 - IOMM-Lee

AU - Lee, W. H.

PY - 1990

Y1 - 1990

N2 - A permanent malignant meningioma (MM) cell line of the human brain designated 'IOMM-Lee' is reported. This cell line was successfully established from the tumor of a 61-year-old Chinese man with repeated recurrent primary intraosseous malignant meningioma of the skull. It has been subcultured for more than 60 passages during the past 30 months. The doubling time of cultured cells is approximately 62 hours. Tumorigenicity in athymic nude mice (Balb/c-nu/nu) who develop multiple pulmonary metastases was observed; the doubling time of tumor volume in vivo is approximately 5 days. Karyotypic analysis revealed this cell line to be of human origin and near-diploid, with a modal chromosome number of 49. The mesenchymal tumor marker vimentin and intracytoplasmic microfilaments were identified in the cytoplasm of tumor cells by indirect immunohistochemical peroxidase-anti-peroxidase assays and immunogold ultrastructural localization by transmission electron microscopy, respectively. SCanning electron microscopy of cultured cells and xenografted tumors revealed ellipsoidal or carrot-shaped tumor cells presenting a wrinkled surface with short sparse microvilli. Potential proliferating activity was determined by Ki-67 monoclonal antibody; the Ki-67 labeling index of cultured cells and xenografted tumors was approximately 36% and 30%, respectively. This newly established malignant meningioma cell line of the human brain may prove useful as a research model.

AB - A permanent malignant meningioma (MM) cell line of the human brain designated 'IOMM-Lee' is reported. This cell line was successfully established from the tumor of a 61-year-old Chinese man with repeated recurrent primary intraosseous malignant meningioma of the skull. It has been subcultured for more than 60 passages during the past 30 months. The doubling time of cultured cells is approximately 62 hours. Tumorigenicity in athymic nude mice (Balb/c-nu/nu) who develop multiple pulmonary metastases was observed; the doubling time of tumor volume in vivo is approximately 5 days. Karyotypic analysis revealed this cell line to be of human origin and near-diploid, with a modal chromosome number of 49. The mesenchymal tumor marker vimentin and intracytoplasmic microfilaments were identified in the cytoplasm of tumor cells by indirect immunohistochemical peroxidase-anti-peroxidase assays and immunogold ultrastructural localization by transmission electron microscopy, respectively. SCanning electron microscopy of cultured cells and xenografted tumors revealed ellipsoidal or carrot-shaped tumor cells presenting a wrinkled surface with short sparse microvilli. Potential proliferating activity was determined by Ki-67 monoclonal antibody; the Ki-67 labeling index of cultured cells and xenografted tumors was approximately 36% and 30%, respectively. This newly established malignant meningioma cell line of the human brain may prove useful as a research model.

KW - cell culture

KW - electron microscopy

KW - immunogold

KW - Ki-67 labeling index

KW - malignant meningioma

KW - nude mice

UR - http://www.scopus.com/inward/record.url?scp=0025144539&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025144539&partnerID=8YFLogxK

M3 - Article

C2 - 2234331

AN - SCOPUS:0025144539

VL - 27

SP - 389

EP - 396

JO - Neurosurgery

JF - Neurosurgery

SN - 0148-396X

IS - 3

ER -