Characterization of a 36 kDa antigenic protein of fish-specific monoclonal-antibody 8F5

Yi Tien Chen, Yun Hwa Peggy Hsieh

研究成果: 雜誌貢獻文章同行評審

摘要

The 36 kDa antigenic protein of a fish-specific monoclonal antibody (mAb), 8F5, previously developed to detect fish in foodstuffs to protect fish allergic individuals, was characterized to establish its identity and to identify the fish-specific epitope. We hypothesized that this antigenic protein is tropomyosin based on its thermal stability and molecular weight. Western blot showed that both the 36 kDa protein and fish tropomyosin were recognized by mAb 8F5, and their molecular weight migration in urea gel electrophoresis was identical. In addition to matching the amino acid composition profile, this 36 kDa protein's sequences precisely correspond to those in fish tropomyosin fragments. Further analysis revealed the sequence of the fish-specific epitope bound by mAb 8F5 to be EDDLVALQKK. These results confirm that the 36 kDa protein is indeed tropomyosin and will be a suitable biomarker for the immunodetection of fish in cooked food.
原文英語
文章編號132149
期刊Food Chemistry
379
DOIs
出版狀態已發佈 - 6月 15 2022

ASJC Scopus subject areas

  • 分析化學
  • 食品科學

指紋

深入研究「Characterization of a 36 kDa antigenic protein of fish-specific monoclonal-antibody 8F5」主題。共同形成了獨特的指紋。

引用此