Characterization of 3-hydroxyisobutyrate dehydrogenase, HIBADH, as a sperm-motility marker

Yung Chieh Tasi, Hsin Chih Albert Chao, Chia Ling Chung, Xiu Ying Liu, Ying Ming Lin, Pao Chi Liao, Hsien An Pan, Han-Sun Chiang, Pao Lin Kuo, Ying Hung Lin

研究成果: 雜誌貢獻文章

14 引文 斯高帕斯(Scopus)

摘要

Purpose: Asthenozoospermia is a major cause of male infertility. However, the molecular mechanisms underlying sperm-motility defects remain largely unknown in the majority of cases. In our previous study, we applied a proteomic approach to identify unknown proteins that were downregulated in spermatozoa with low motility compared to spermatozoa with good motility. Several sperm motility- related proteins have been identified. In this study, 3-hydroxyisobutyrate dehydrogenase (HIBADH), one of the proteins identified using the proteomic tools, is further characterized. Methods: Reverse-transcription polymerase chain reactions (RT-PCR), western blotting, and immunofluorescence assays (IFA) were preformed to investigate the expression pattern. The enzymatic activity of HIBADH was evaluated in sperm with good (>50 %), moderate (<50 %) and lower motility (<20 %). Results: Using RT-PCR, we found that transcripts of HIBADH are enriched in the cerebellum, heart, skeletal muscle, uterus, placenta, and testes of male humans. In western blotting, it is expressed in the placenta, testes, and spermatozoa. During spermiogenesis, HIBADH is located at the mid-piece (a specialized development from the mitochondria) of elongating, elongated, and mature sperm. The enzymatic activity of HIBADH in sperm with moderate and lower motility were significantly reduced compared with good motility (P <0.0001 and P <0.05, respectively). Conclusions: Our study indicated that HIBADH is involved in the mitochondrial function of spermatozoa, and maintains sperm motility. It may serve as a sperm-motility marker.
原文英語
頁(從 - 到)1-8
頁數8
期刊Journal of Assisted Reproduction and Genetics
30
發行號4
DOIs
出版狀態接受/付印 - 2013
對外發佈Yes

ASJC Scopus subject areas

  • Reproductive Medicine
  • Obstetrics and Gynaecology
  • Genetics(clinical)
  • Genetics
  • Developmental Biology

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    Tasi, Y. C., Chao, H. C. A., Chung, C. L., Liu, X. Y., Lin, Y. M., Liao, P. C., Pan, H. A., Chiang, H-S., Kuo, P. L., & Lin, Y. H. (認可的出版社/出版中). Characterization of 3-hydroxyisobutyrate dehydrogenase, HIBADH, as a sperm-motility marker. Journal of Assisted Reproduction and Genetics, 30(4), 1-8. https://doi.org/10.1007/s10815-013-9954-8