Characterization of γ-Crystallins from a Hybrid Teleostean Fish: Multiplicity of Isoforms as Revealed by cDNA Sequence Analysis

F. M. Pan, W. C. Chang, Y. K. Chao, S. H. Chiou

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13 引文 斯高帕斯(Scopus)

摘要

γ-Crystallins were isolated and characterized from the eye lenses of a hybrid species belonging to the teleostean fish. Isoelectric focusing of γ-crystallin fraction obtained from gel-permeation chromatography revealed that it consists of multiple charge isomers of a protein species with a molecular mass of about 20 kDa. To facilitate the cloning of γ-crystallin gene, cDNA was constructed from the poly(A)+mRNA isolated from fresh lenses, and amplification by polymerase chain reaction (PCR) was carried out to obtain cDNA encoding multiple γ-crystallins. Sequencing five of more than 10 positive clones revealed that a multiplicity of isoforms exists in the γ-crystallin class of teleostean lenses. Comparison of protein sequences encoded by these multiple cDNAs with those published sequences of γ-crystallins from bovine, mouse and carp lenses indicated that there is about 70-80 % sequence homology between different species of piscine species whereas only 50-60 % is found between mammals and fishes. Structural analysis of these γ-crystallins with high methionine contents (11-16 %) suggests that there are two major subclasses of piscine γ-crystallins, i.e. γM1 and γM2, existed long before the appearance of mammalian γ-crystallin with low methionines.
原文英語
頁(從 - 到)527-534
頁數8
期刊Biochemical and Biophysical Research Communications
202
發行號1
DOIs
出版狀態已發佈 - 七月 15 1994
對外發佈

ASJC Scopus subject areas

  • 生物物理學
  • 生物化學
  • 分子生物學
  • 細胞生物學

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