Characterisation of antibodies to bovine toll-like receptor (TLR)-2 and cross-reactivity with ovine TLR2

Lai Shan Kwong, Richard Parsons, Robert Patterson, Tracey J. Coffey, Leenadevi Thonur, Jung Su Chang, George Russell, David Haig, Dirk Werling, Jayne C. Hope

研究成果: 雜誌貢獻文章同行評審

10 引文 斯高帕斯(Scopus)


Host recognition of conserved pathogen-associated molecular patterns (PAMPs) and their interactions with pattern-recognition receptors, including the Toll-like receptors (TLR) is essential for innate immune response induction. The TLR1 family (TLR1, 2, 6 and 10) is involved in the recognition of gram-positive and gram-negative bacteria and heterodimers of TLR1 or TLR6 with TLR2 are crucial for the identification of several PAMPs.Studies on cell surface expression of TLR in ruminants are hampered by the lack of specific antibodies and no convincingly cross-reactive anti-human antibodies have been described so far. We describe herein four antibodies which recognise bovine TLR2. Differences in TLR2 expression were evident on bovine antigen presenting cells with high level expression on peripheral blood monocytes and monocyte-derived macrophages. Lower levels of expression were evident on dendritic cell populations derived in vitro and ex vivo, and on alveolar macrophages. One of the antibodies recognised TLR2 expression on ovine peripheral blood monocytes. The identification of antibodies specific for bovine and ovine TLR2 will facilitate studies of the role of this important PRR in the initiation of immune responses to important pathogens.
頁(從 - 到)313-318
期刊Veterinary Immunology and Immunopathology
出版狀態已發佈 - 2月 15 2011

ASJC Scopus subject areas

  • 免疫學
  • 獸醫 (全部)


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