Changes of superoxide dismutase (SOD) mRNA and activity in response to hypoxic stress in cultured Wistar rat glioma cells

Cheng Kuei Chang, Chung Yau Tsai, Lieh Sheng Lin, Shuo Bin Jou, Shue Sen Liao, Juei Tang Cheng

研究成果: 雜誌貢獻文章

12 引文 (Scopus)

摘要

In an attempt to understand the change of superoxide dismutase (SOD) in tumor cells by hypoxia and hypoxia-normoxia exposure, the present study performed an in vitro investigation using rat glioma cell line in culture. Hypoxia was induced by an incubation with nitrogen gas for 15 h followed the normoxia exposure with air for 30 min. Activity of SOD in cytosolic and particulate of cells was determined by the reduction of nitroblue tetrazolium. Changes of mRNA for Cu,Zn-SOD or Mn-SOD were also characterized using Northern blotting analysis. Hypoxic stress decreased the activity of SOD, both Cu,Zn-SOD and Mn-SOD, in glioma cells. Expression of mRNA for SOD was elevated by hypoxic stress and the increase of mRNA level for Cu,Zn-SOD was more marked than that for Mn-SOD. In response to hypoxia-normoxia exposure, an increase of activity with a lower mRNA level for Mn-SOD was observed in glioma cells. However, changes of Cu,Zn-SOD both the activity and the level of mRNA were not found in glioma cells by hypoxia-normoxia. The obtained results suggest that the SOD in glioma cells can be activated to compensate the damage from free radicals during hypoxic stress.
原文英語
頁(從 - 到)115-118
頁數4
期刊Neuroscience Letters
232
發行號2
DOIs
出版狀態已發佈 - 八月 29 1997
對外發佈Yes

指紋

Glioma
Superoxide Dismutase
Wistar Rats
Messenger RNA
Nitroblue Tetrazolium
Cell Hypoxia
Northern Blotting
Free Radicals
Nitrogen
Gases
Air
Cell Line
Superoxide Dismutase-1
Hypoxia

ASJC Scopus subject areas

  • Neuroscience(all)

引用此文

Changes of superoxide dismutase (SOD) mRNA and activity in response to hypoxic stress in cultured Wistar rat glioma cells. / Chang, Cheng Kuei; Tsai, Chung Yau; Lin, Lieh Sheng; Jou, Shuo Bin; Liao, Shue Sen; Cheng, Juei Tang.

於: Neuroscience Letters, 卷 232, 編號 2, 29.08.1997, p. 115-118.

研究成果: 雜誌貢獻文章

Chang, Cheng Kuei ; Tsai, Chung Yau ; Lin, Lieh Sheng ; Jou, Shuo Bin ; Liao, Shue Sen ; Cheng, Juei Tang. / Changes of superoxide dismutase (SOD) mRNA and activity in response to hypoxic stress in cultured Wistar rat glioma cells. 於: Neuroscience Letters. 1997 ; 卷 232, 編號 2. 頁 115-118.
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abstract = "In an attempt to understand the change of superoxide dismutase (SOD) in tumor cells by hypoxia and hypoxia-normoxia exposure, the present study performed an in vitro investigation using rat glioma cell line in culture. Hypoxia was induced by an incubation with nitrogen gas for 15 h followed the normoxia exposure with air for 30 min. Activity of SOD in cytosolic and particulate of cells was determined by the reduction of nitroblue tetrazolium. Changes of mRNA for Cu,Zn-SOD or Mn-SOD were also characterized using Northern blotting analysis. Hypoxic stress decreased the activity of SOD, both Cu,Zn-SOD and Mn-SOD, in glioma cells. Expression of mRNA for SOD was elevated by hypoxic stress and the increase of mRNA level for Cu,Zn-SOD was more marked than that for Mn-SOD. In response to hypoxia-normoxia exposure, an increase of activity with a lower mRNA level for Mn-SOD was observed in glioma cells. However, changes of Cu,Zn-SOD both the activity and the level of mRNA were not found in glioma cells by hypoxia-normoxia. The obtained results suggest that the SOD in glioma cells can be activated to compensate the damage from free radicals during hypoxic stress.",
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