Changes of Protein Kinase C Subspecies in Staphylococcal Enterotoxin B-Induced Thymocyte Apoptosis

Y. S. Lin, S. F. Kao, M. S. Jan, M. L. Cheng, L. Y C Wing, W. C. Chang, H. Y. Lei, M. T. Lin

研究成果: 雜誌貢獻文章

10 引文 斯高帕斯(Scopus)

摘要

Our previous studies demonstrated that intravenous administration of staphylococcal enterotoxin B (SEB) to BALB/c mice resulted in thymocyte apoptosis. In the present study, we evaluated the role of protein kinase C (PKC) in thymocyte apoptosis induced by SEB. Our results showed that the level of-protein phosphorylation in the thymocytes was reduced after the in vivo SEB treatment for 24 h. The activity of classical PKC subspecies was decreased in both cytosolic and membrane fractions of thymocytes following SEB administration. The lowest level of PKC activity was reached by 24 and 48 h, then was recovered gradually after 72 h. Furthermore, the mRNA expression of PKC-β and, to a much less extent, PKC-α, but not PKC-ζ, in thymocytes was reduced by SEB. The decrease of mRNA level of PKC-β showed good correlation with the pattern of PKC activity. These results provide direct evidence showing the changes in PKC subspecies mRNA expression during the process of apoptosis.

原文英語
頁(從 - 到)1132-1139
頁數8
期刊Biochemical and Biophysical Research Communications
213
發行號3
DOIs
出版狀態已發佈 - 八月 24 1995
對外發佈Yes

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology
  • Biophysics
  • Biochemistry

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