Changes in the mRNA levels of delayed rectifier potassium channels in human atrial fibrillation

Ling Ping Lai, Ming Jai Su, Jiunn Lee Lin, Fang Yue Lin, Chang Her Tsai, Yih Sharng Chen, Yung Zu Tseng, Wen Pin Lien, Shoei K. Stephen Huang

研究成果: 雜誌貢獻文章

43 引文 斯高帕斯(Scopus)

摘要

Introduction: We measured mRNA levels of delayed rectifier potassium channels in human atrial tissue to investigate the mechanism of the shortening of the atrial effective refractory period and the loss of rate-adaptive shortening of the atrial effective refractory period in human atrial fibrillation. Methods and Results: A total of 34 patients undergoing open heart surgery were included. Atrial tissue was obtained from the right atrial free wall, right atrial appendage, left atrial free wall and left atrial appendage, respectively. The mRNA amounts of K(VLQT1) (I(Ks)), minK (β-subunit of I(Ks)), HERG (I(Kr)), and K(V1.5) (I(Kur)) were measured by reverse transcription-polymerase chain reaction and normalized to the mRNA amount of GAPDH. We found that the mRNA levels of K(V1.5), HERG and K(VLQT1) were all significantly decreased in patients with persistent atrial fibrillation for more than 3 months. In contrast, the mRNA level of minK was significantly increased in patients with persistent atrial fibrillation for more than 3 months. We further showed that these changes were independent of the underlying cardiac disease, atrial filling pressure, gender and age. We also found that there was no spatial dispersion of mRNA levels among the four atrial sampling sites. Conclusions: Because the decrease in potassium currents results in a prolonged action potential, the shortening of the atrial effective refractory period in atrial fibrillation should be attributed to other factors. However, the decrease in I(Ks) might contribute, at least in part, to the loss of rate-adaptive shortening of the atrial refractory period. Copyright (C) 2000 S. Karger AG, Basel.
原文英語
頁(從 - 到)248-255
頁數8
期刊Cardiology
92
發行號4
DOIs
出版狀態已發佈 - 一月 1 1999
對外發佈Yes

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Pharmacology (medical)

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    Lai, L. P., Su, M. J., Lin, J. L., Lin, F. Y., Tsai, C. H., Chen, Y. S., Tseng, Y. Z., Lien, W. P., & Stephen Huang, S. K. (1999). Changes in the mRNA levels of delayed rectifier potassium channels in human atrial fibrillation. Cardiology, 92(4), 248-255. https://doi.org/10.1159/000006982