Central serotonergic lesions increase voluntary alcohol consumption in Sprague Dawley rats: Moderation by long-term ethanol administration

Jia Yi Wang, Andrew Y C Shum, Tzu Chao Lin, Yun Wang

研究成果: 雜誌貢獻文章同行評審

12 引文 斯高帕斯(Scopus)

摘要

The relationship between central serotonergic activities and voluntary alcohol consumption was studied in Sprague-Dawley rats, which normally have low alcohol preference. After initial screening for an evenly matched baseline alcohol preference, selective central serotonergic lesioning was induced by intracisternal injection of the serotonergic neurotoxin 5,7- dihydroxytryptamine (5,7-DHT). Control rata received injections of vehicle only. Both 5,7-DHT and vehicle-treated rata were further divided into two subgroups, which either had continued free access to ethanol (alcohol- drinking) or were deprived of it (alcohol-free). All rats were then tested again for alcohol preference. All rats were then killed, and the levels of monoamines in the brains were determined by high performance liquid chromatography with electrochemical detection. Behavioral results indicated that all 5,7-DHT-treated rats had significantly higher alcohol preference and consumption than the corresponding sham controls. Except in the cerebellum, the 5,7-DHT-treated rats had significantly lower levels of serotonin (5-HT) and 5-hydroxyindoleacetic acid (5-HIAA) in most brain regions compared with those in the corresponding sham controls. Treatment with 5,7-DHT also resulted in a decrease in serotonin turnover in all brain regions in the alcohol-free rats, except in the cerebellum. In alcohol-drinking rats, however, 5,7-DHT treatment only reduced serotonin turnover in the pons. The levels of norepinephrine and dopamine in several brain regions were not significantly different. Thus, it appeared that in the Sprague Dawley rats, 5,7-DHT treatment depleted 5-HT and 5-HIAA levels in most brain regions while increasing alcohol consumption. Chronic alcohol-drinking attenuated the increase in alcohol consumption associated with serotonergic lesions. Voluntary alcohol consumption seemed more associated with 5-HT turnover than with tissue 5-HT levels. Our data also suggested that tolerance to alcohol- induced hypothermia was primarily attributable to long-term alcohol drinking rather than serotonergic lesioning.
原文英語
頁(從 - 到)1252-1259
頁數8
期刊Alcoholism: Clinical and Experimental Research
20
發行號7
DOIs
出版狀態已發佈 - 1996
對外發佈

ASJC Scopus subject areas

  • 醫藥(雜項)
  • 毒理學

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