Cellular photodynamic toxicity of hematoporphyrin in various nanocarrier systems

Yu Tsai Yang, Chin Tin Chen, Hsiung-Fei Chien, Tsui-Min Tsai

研究成果: 雜誌貢獻文章同行評審

1 引文 斯高帕斯(Scopus)

摘要

In this study, we investigated the cellular uptake of Hematoporphyrin (Hp) encapsulated in nanocarriers by human lung adenocarcinoma A549 cells and the photodynamic toxic effects on those cells. The nanocarriers included liposomes, micelles, and nanoparticles. The Hp was encapsulated in liposomes through a film hydration and extrusion method. Hp was encapsulated in micelles through a film hydration method. The Hp loaded nanoparticles were prepared using an emulsification-diffusion process. The Hp in nanocarriers was characterized by laser light scattering to determine particle size and zeta potential of the surface charge. The cellular uptake of nanocarriers was measured by fluorescence spectrometry. The photodynamic toxicity of various Hp nanocarriers was evaluated in human lung epithelial carcinoma A549 cells using a light-emitting diode (LED) device at a wavelength of 630±5 nm. The averaged size of all nanocarriers was between 112 and 135 nm. The surface charge observed for all formulations, particularly the micelles showed lower negative valued than those of other systems. Cellular uptake and photocytotoxicity of the four Hp preparations in serum free medium were in the following order: micelle-Hp > nanoparticle-Hp > liposome-Hp > free Hp. In the presence of serum protein, the photodynamic toxicity of all formulations significantly decreased.
原文英語
頁(從 - 到)850-855
頁數6
期刊Current Nanoscience
7
發行號6
DOIs
出版狀態已發佈 - 十二月 2011

ASJC Scopus subject areas

  • 生物技術
  • 生物工程
  • 生物醫學工程
  • 醫藥(雜項)
  • 藥學科學

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