Cell surface expression of a specific antigenic site on the catalytic subunit of (Na+ + K+)-ATPase

Allen C. Myers; Bruce S. Bochner; Gordon F. Tomaselli; Neal Fedarko; Sherry A. Hudson; Holly Rohde; Shau Ku Huang; Kai Y. Xu

doi:10.1006/bbrc.2002.6419

Cell surface expression of a specific antigenic site on the catalytic subunit of (Na⁺ + K⁺)-ATPase

Allen C. Myers, Bruce S. Bochner, Gordon F. Tomaselli, Neal Fedarko, Sherry A. Hudson, Holly Rohde, Shau Ku Huang, Kai Y. Xu

醫學檢驗暨生物技術學系所

研究成果: 雜誌貢獻 › 文章 › 同行評審

2 引文斯高帕斯（Scopus）

摘要

Structural localization of a peptide region, KRQPRNPKTDKLVNE, in the catalytic subunit of (Na⁺ + K⁺)-ATPase was investigated using a specific antibody directed against this peptide in cultured African green monkey kidney CV-1 cells. Immunofluorescence staining of frozen cell sections shows that an anti-KRQPRNPKTDKLVNE antibody (SSA95) interacts with its antigenic site and binds to the extracellular side of the cell membrane. Indirect immunofluorescence and flow cytometric analyses confirmed the presence of this epitope on intact cell surfaces. These results suggest that the KRQPRNPKTDKLVNE region of the (Na⁺ + K⁺)-ATPase is expressed on the cellular membrane surface.

原文	英語
頁（從 - 到）	111-115
頁數	5
期刊	Biochemical and Biophysical Research Communications
卷	291
發行號	1
DOIs	https://doi.org/10.1006/bbrc.2002.6419
出版狀態	已發佈 - 2002

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1006/bbrc.2002.6419

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Cell surface expression of a specific antigenic site on the catalytic subunit of (Na⁺ + K⁺)-ATPase. / Myers, Allen C.; Bochner, Bruce S.; Tomaselli, Gordon F.; Fedarko, Neal; Hudson, Sherry A.; Rohde, Holly; Huang, Shau Ku; Xu, Kai Y.

於: Biochemical and Biophysical Research Communications, 卷 291, 編號 1, 2002, p. 111-115.

研究成果: 雜誌貢獻 › 文章 › 同行評審

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abstract = "Structural localization of a peptide region, KRQPRNPKTDKLVNE, in the catalytic subunit of (Na+ + K+)-ATPase was investigated using a specific antibody directed against this peptide in cultured African green monkey kidney CV-1 cells. Immunofluorescence staining of frozen cell sections shows that an anti-KRQPRNPKTDKLVNE antibody (SSA95) interacts with its antigenic site and binds to the extracellular side of the cell membrane. Indirect immunofluorescence and flow cytometric analyses confirmed the presence of this epitope on intact cell surfaces. These results suggest that the KRQPRNPKTDKLVNE region of the (Na+ + K+)-ATPase is expressed on the cellular membrane surface.",

keywords = "(Na + K)-ATPase, Flow cytometry, Immunofluorescence staining, Positive inotropy, Site-specific antibody",

author = "Myers, {Allen C.} and Bochner, {Bruce S.} and Tomaselli, {Gordon F.} and Neal Fedarko and Hudson, {Sherry A.} and Holly Rohde and Huang, {Shau Ku} and Xu, {Kai Y.}",

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T1 - Cell surface expression of a specific antigenic site on the catalytic subunit of (Na+ + K+)-ATPase

AU - Myers, Allen C.

AU - Bochner, Bruce S.

AU - Tomaselli, Gordon F.

AU - Fedarko, Neal

AU - Hudson, Sherry A.

AU - Rohde, Holly

AU - Huang, Shau Ku

AU - Xu, Kai Y.

PY - 2002

Y1 - 2002

N2 - Structural localization of a peptide region, KRQPRNPKTDKLVNE, in the catalytic subunit of (Na+ + K+)-ATPase was investigated using a specific antibody directed against this peptide in cultured African green monkey kidney CV-1 cells. Immunofluorescence staining of frozen cell sections shows that an anti-KRQPRNPKTDKLVNE antibody (SSA95) interacts with its antigenic site and binds to the extracellular side of the cell membrane. Indirect immunofluorescence and flow cytometric analyses confirmed the presence of this epitope on intact cell surfaces. These results suggest that the KRQPRNPKTDKLVNE region of the (Na+ + K+)-ATPase is expressed on the cellular membrane surface.

AB - Structural localization of a peptide region, KRQPRNPKTDKLVNE, in the catalytic subunit of (Na+ + K+)-ATPase was investigated using a specific antibody directed against this peptide in cultured African green monkey kidney CV-1 cells. Immunofluorescence staining of frozen cell sections shows that an anti-KRQPRNPKTDKLVNE antibody (SSA95) interacts with its antigenic site and binds to the extracellular side of the cell membrane. Indirect immunofluorescence and flow cytometric analyses confirmed the presence of this epitope on intact cell surfaces. These results suggest that the KRQPRNPKTDKLVNE region of the (Na+ + K+)-ATPase is expressed on the cellular membrane surface.

KW - (Na + K)-ATPase

KW - Flow cytometry

KW - Immunofluorescence staining

KW - Positive inotropy

KW - Site-specific antibody

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