Cell fractionation on pH-responsive chitosan surface

Yi Hsin Chen, Shao Hsuan Chang, Tsung Jen Wang, I. Jong Wang, Tai Horng Young

研究成果: 雜誌貢獻文章

7 引文 (Scopus)

摘要

The purpose of this study is to demonstrate pH-responsive chitosan is able to be used for cell fractionation under precise adjustment of medium pH. Cells were first seeded to attach on chitosan surface at medium pH 7.20 for 24 h. After raising medium pH to 7.65 for 1 h, cells with elongated morphology possessed rapider detachment rate and cells with round shape detached at a lesser rate. Therefore, successful cell separation has been achieved by choosing appropriate cell combination with different detachment rates without additional antibody or enzyme treatment and extensive washing steps. Furthermore, the method also could be applied to specific manipulation of viable cell populations from tissue specimen. Most importantly and interestingly, the efficiency of cell fractionation of our system could be theoretically predicted according to the individual cell detachment rate on pH-responsive chitosan surface, without considering the presence of heterotypic cells.

原文英語
頁(從 - 到)854-863
頁數10
期刊Biomaterials
34
發行號4
DOIs
出版狀態已發佈 - 一月 2013

指紋

Cell Fractionation
Chitosan
Fractionation
Antibodies
Washing
Enzymes
Cells
Tissue
Cell Separation

ASJC Scopus subject areas

  • Biomaterials
  • Bioengineering
  • Ceramics and Composites
  • Mechanics of Materials
  • Biophysics

引用此文

Cell fractionation on pH-responsive chitosan surface. / Chen, Yi Hsin; Chang, Shao Hsuan; Wang, Tsung Jen; Wang, I. Jong; Young, Tai Horng.

於: Biomaterials, 卷 34, 編號 4, 01.2013, p. 854-863.

研究成果: 雜誌貢獻文章

Chen, YH, Chang, SH, Wang, TJ, Wang, IJ & Young, TH 2013, 'Cell fractionation on pH-responsive chitosan surface', Biomaterials, 卷 34, 編號 4, 頁 854-863. https://doi.org/10.1016/j.biomaterials.2012.10.014
Chen, Yi Hsin ; Chang, Shao Hsuan ; Wang, Tsung Jen ; Wang, I. Jong ; Young, Tai Horng. / Cell fractionation on pH-responsive chitosan surface. 於: Biomaterials. 2013 ; 卷 34, 編號 4. 頁 854-863.
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