Cell cycle-dependent localization of the CDK2-cyclin E complex in Cajal (coiled) bodies

Juinn Lin Liu, Michael D. Hebert, Ying Ye, Dennis J. Templeton, Hsing Jien Kung, A. Gregory Matera

研究成果: 雜誌貢獻文章

56 引文 (Scopus)

摘要

We have found that CDK2 and cyclin E, but not cyclin A, accumulates within Cajal bodies (CBs) in a cell cycle-dependent fashion. In the absence of cyclin E, CDK2 is not enriched in the CB compartment, suggesting that the translocation of CDK2 to CBs is dependent on cyclin E. CDK2 and cyclin E could be recruited to CBs as a functional complex or CBs may serve as 'docking stations' for CDK2-cyclin E activation by CAKs during the G1/S transition. Notably, CDK7-cyclin H-Mat1 complexes are known to accumulate in CBs. Treatment of cells with inhibitors of either CDKs (olomoucine, 200 μM) or RNA polymerase I (actinomycin D, 0.05 μg/ml), results in a striking reorganization of CDK2 and p80 coilin to the nucleolar periphery. Furthermore, we demonstrate that p80 coilin can be phosphorylated by purified CDK2-cyclin E complexes in vitro. Thus coilin and other CB proteins appear to be downstream targets of CDK2-cyclin E complex-mediated signaling pathways regulating cell cycle progression and controlling aspects of CB function. Possible roles for CDK2 and cyclin E in the well-documented association of CBs, histone gene clusters and RNA 3' end processing factors are discussed.
原文英語
頁(從 - 到)1543-1552
頁數10
期刊Journal of Cell Science
113
發行號9
出版狀態已發佈 - 六月 5 2000
對外發佈Yes

指紋

Coiled Bodies
Cyclin E
Cell Cycle
RNA 3' End Processing
Cyclin H
RNA Polymerase I
Cyclin A
Dactinomycin
Multigene Family
Histones

ASJC Scopus subject areas

  • Cell Biology

引用此文

Liu, J. L., Hebert, M. D., Ye, Y., Templeton, D. J., Kung, H. J., & Matera, A. G. (2000). Cell cycle-dependent localization of the CDK2-cyclin E complex in Cajal (coiled) bodies. Journal of Cell Science, 113(9), 1543-1552.

Cell cycle-dependent localization of the CDK2-cyclin E complex in Cajal (coiled) bodies. / Liu, Juinn Lin; Hebert, Michael D.; Ye, Ying; Templeton, Dennis J.; Kung, Hsing Jien; Matera, A. Gregory.

於: Journal of Cell Science, 卷 113, 編號 9, 05.06.2000, p. 1543-1552.

研究成果: 雜誌貢獻文章

Liu, JL, Hebert, MD, Ye, Y, Templeton, DJ, Kung, HJ & Matera, AG 2000, 'Cell cycle-dependent localization of the CDK2-cyclin E complex in Cajal (coiled) bodies', Journal of Cell Science, 卷 113, 編號 9, 頁 1543-1552.
Liu JL, Hebert MD, Ye Y, Templeton DJ, Kung HJ, Matera AG. Cell cycle-dependent localization of the CDK2-cyclin E complex in Cajal (coiled) bodies. Journal of Cell Science. 2000 6月 5;113(9):1543-1552.
Liu, Juinn Lin ; Hebert, Michael D. ; Ye, Ying ; Templeton, Dennis J. ; Kung, Hsing Jien ; Matera, A. Gregory. / Cell cycle-dependent localization of the CDK2-cyclin E complex in Cajal (coiled) bodies. 於: Journal of Cell Science. 2000 ; 卷 113, 編號 9. 頁 1543-1552.
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