摘要
原文 | 英語 |
---|---|
頁(從 - 到) | 153-61 |
頁數 | 9 |
期刊 | Blood |
卷 | 38 |
發行號 | 2 |
出版狀態 | 已發佈 - 八月 1971 |
對外發佈 | Yes |
指紋
引用此文
Cell cycle characteristics, maturation, and phagocytosis in vitro of blast cells from patients with chronic myelocytic leukemia. / Whang-Peng, J; Perry, S; Knutsen, T A; Gart, J J.
於: Blood, 卷 38, 編號 2, 08.1971, p. 153-61.研究成果: 雜誌貢獻 › 文章
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TY - JOUR
T1 - Cell cycle characteristics, maturation, and phagocytosis in vitro of blast cells from patients with chronic myelocytic leukemia
AU - Whang-Peng, J
AU - Perry, S
AU - Knutsen, T A
AU - Gart, J J
PY - 1971/8
Y1 - 1971/8
N2 - Cell cycle characteristics, phagocytosis and maturation of immature white blood cells from seven patients with Ph1-positive chronic myelocytic leukemia were studied in vitro. In five patients whose cells contained only one Ph1 chromosome there were two in early stable phase, one in transitional phase, and two in blastic crisis. The other two patients had cell lines with more than one Ph1 chromosome in addition to cells containing one Ph1 chromosome. Cell cycle times in the seven patients as determined with tritiated thymidine ranged from 48 to 59 hr regardless of stage of the disease with one exception (32 hr in a patient who died within 1 wk). A small percentage of myeloblasts was arrested in early S or G2 for the duration of the study of up to 1 wk. Maturation in vitro was observed with appearance of the first labeled metamyelocytes at 2 hr and first labeled mature segmented and nonsegmented neutrophils at 10 hr (with the median time of 22 hr of in vitro culture). Results of this study suggest that cell precursors in blastic transformation have a diminished capability to mature despite the early presence of mature neutrophils in vitro. Early appearance of labeled mature neutrophils and irregularity of the time sequence are characteristic of cells from patients in blastic transformation. Immature granulocytic precursors were shown capable of phagocytosis in vitro, suggesting that although nuclear maturation was delayed, membrane and cytoplasmic function was intact. This dissociation may be characteristic of leukocytes from patients with chronic myelocytic leukemia.
AB - Cell cycle characteristics, phagocytosis and maturation of immature white blood cells from seven patients with Ph1-positive chronic myelocytic leukemia were studied in vitro. In five patients whose cells contained only one Ph1 chromosome there were two in early stable phase, one in transitional phase, and two in blastic crisis. The other two patients had cell lines with more than one Ph1 chromosome in addition to cells containing one Ph1 chromosome. Cell cycle times in the seven patients as determined with tritiated thymidine ranged from 48 to 59 hr regardless of stage of the disease with one exception (32 hr in a patient who died within 1 wk). A small percentage of myeloblasts was arrested in early S or G2 for the duration of the study of up to 1 wk. Maturation in vitro was observed with appearance of the first labeled metamyelocytes at 2 hr and first labeled mature segmented and nonsegmented neutrophils at 10 hr (with the median time of 22 hr of in vitro culture). Results of this study suggest that cell precursors in blastic transformation have a diminished capability to mature despite the early presence of mature neutrophils in vitro. Early appearance of labeled mature neutrophils and irregularity of the time sequence are characteristic of cells from patients in blastic transformation. Immature granulocytic precursors were shown capable of phagocytosis in vitro, suggesting that although nuclear maturation was delayed, membrane and cytoplasmic function was intact. This dissociation may be characteristic of leukocytes from patients with chronic myelocytic leukemia.
KW - Autoradiography
KW - Bone Marrow
KW - Bone Marrow Cells
KW - Cell Differentiation
KW - Cell Membrane
KW - Cell Nucleus
KW - Cell Transformation, Neoplastic
KW - Chromosome Aberrations
KW - Culture Techniques
KW - Cytoplasm
KW - Female
KW - Hematopoietic Stem Cells
KW - Humans
KW - Karyotyping
KW - Leukemia, Myeloid
KW - Male
KW - Mitosis
KW - Phagocytosis
KW - Thymidine
KW - Tritium
KW - Journal Article
M3 - Article
C2 - 5283983
VL - 38
SP - 153
EP - 161
JO - Blood
JF - Blood
SN - 0006-4971
IS - 2
ER -