Cell-based semiquantitative assay for sulfated glycosaminoglycans facilitating the identification of chondrogenesis

Ching Yu Yen, Yu Wei Wu, Chao Nan Hsiung, Min I. Yeh, Yi Ming Lin, Sheng Yang Lee

研究成果: 雜誌貢獻文章

摘要

Glycosaminoglycans (GAGs), in particular chondroitin sulfate, are an accepted marker of chondrogenic cells. In this study, a cell-based sulfated GAG assay for identifying the chondrogenesis of mesenchymal stem cells was developed. Based on fluorescent staining using safranin O and 4',6-diamidino-2-phenylindole (DAPI), this method was highly sensitive. The results were both qualitative and quantitative. The method is suitable for identifying the chondrogenic process and also for screening compounds. The method may be helpful for discovering novel bioactive compounds for cartilage regeneration.
原文英語
頁(從 - 到)41-43
頁數3
期刊Analytical Biochemistry
486
DOIs
出版狀態已發佈 - 七月 21 2015

指紋

A73025
Chondrogenesis
Chondroitin Sulfates
Cartilage
Glycosaminoglycans
Stem cells
Assays
Screening
Mesenchymal Stromal Cells
Regeneration
Staining and Labeling
safranine T
DAPI

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology
  • Cell Biology

引用此文

Cell-based semiquantitative assay for sulfated glycosaminoglycans facilitating the identification of chondrogenesis. / Yen, Ching Yu; Wu, Yu Wei; Hsiung, Chao Nan; Yeh, Min I.; Lin, Yi Ming; Lee, Sheng Yang.

於: Analytical Biochemistry, 卷 486, 21.07.2015, p. 41-43.

研究成果: 雜誌貢獻文章

@article{f7f68bee3032419eb3d62b78ad85a8b0,
title = "Cell-based semiquantitative assay for sulfated glycosaminoglycans facilitating the identification of chondrogenesis",
abstract = "Glycosaminoglycans (GAGs), in particular chondroitin sulfate, are an accepted marker of chondrogenic cells. In this study, a cell-based sulfated GAG assay for identifying the chondrogenesis of mesenchymal stem cells was developed. Based on fluorescent staining using safranin O and 4',6-diamidino-2-phenylindole (DAPI), this method was highly sensitive. The results were both qualitative and quantitative. The method is suitable for identifying the chondrogenic process and also for screening compounds. The method may be helpful for discovering novel bioactive compounds for cartilage regeneration.",
keywords = "Chondrogenesis, DAPI, Glycosaminoglycan, Safranin O, Screening",
author = "Yen, {Ching Yu} and Wu, {Yu Wei} and Hsiung, {Chao Nan} and Yeh, {Min I.} and Lin, {Yi Ming} and Lee, {Sheng Yang}",
year = "2015",
month = "7",
day = "21",
doi = "10.1016/j.ab.2015.06.005",
language = "English",
volume = "486",
pages = "41--43",
journal = "Analytical Biochemistry",
issn = "0003-2697",
publisher = "Academic Press Inc.",

}

TY - JOUR

T1 - Cell-based semiquantitative assay for sulfated glycosaminoglycans facilitating the identification of chondrogenesis

AU - Yen, Ching Yu

AU - Wu, Yu Wei

AU - Hsiung, Chao Nan

AU - Yeh, Min I.

AU - Lin, Yi Ming

AU - Lee, Sheng Yang

PY - 2015/7/21

Y1 - 2015/7/21

N2 - Glycosaminoglycans (GAGs), in particular chondroitin sulfate, are an accepted marker of chondrogenic cells. In this study, a cell-based sulfated GAG assay for identifying the chondrogenesis of mesenchymal stem cells was developed. Based on fluorescent staining using safranin O and 4',6-diamidino-2-phenylindole (DAPI), this method was highly sensitive. The results were both qualitative and quantitative. The method is suitable for identifying the chondrogenic process and also for screening compounds. The method may be helpful for discovering novel bioactive compounds for cartilage regeneration.

AB - Glycosaminoglycans (GAGs), in particular chondroitin sulfate, are an accepted marker of chondrogenic cells. In this study, a cell-based sulfated GAG assay for identifying the chondrogenesis of mesenchymal stem cells was developed. Based on fluorescent staining using safranin O and 4',6-diamidino-2-phenylindole (DAPI), this method was highly sensitive. The results were both qualitative and quantitative. The method is suitable for identifying the chondrogenic process and also for screening compounds. The method may be helpful for discovering novel bioactive compounds for cartilage regeneration.

KW - Chondrogenesis

KW - DAPI

KW - Glycosaminoglycan

KW - Safranin O

KW - Screening

UR - http://www.scopus.com/inward/record.url?scp=84937611156&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84937611156&partnerID=8YFLogxK

U2 - 10.1016/j.ab.2015.06.005

DO - 10.1016/j.ab.2015.06.005

M3 - Article

C2 - 26070853

AN - SCOPUS:84937611156

VL - 486

SP - 41

EP - 43

JO - Analytical Biochemistry

JF - Analytical Biochemistry

SN - 0003-2697

ER -