Cell-based semiquantitative assay for sulfated glycosaminoglycans facilitating the identification of chondrogenesis

Ching Yu Yen, Yu Wei Wu, Chao Nan Hsiung, Min I. Yeh, Yi Ming Lin, Sheng Yang Lee

研究成果: 雜誌貢獻文章

摘要

Glycosaminoglycans (GAGs), in particular chondroitin sulfate, are an accepted marker of chondrogenic cells. In this study, a cell-based sulfated GAG assay for identifying the chondrogenesis of mesenchymal stem cells was developed. Based on fluorescent staining using safranin O and 4',6-diamidino-2-phenylindole (DAPI), this method was highly sensitive. The results were both qualitative and quantitative. The method is suitable for identifying the chondrogenic process and also for screening compounds. The method may be helpful for discovering novel bioactive compounds for cartilage regeneration.
原文英語
頁(從 - 到)41-43
頁數3
期刊Analytical Biochemistry
486
DOIs
出版狀態已發佈 - 七月 21 2015

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology
  • Cell Biology

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