CDK2 phosphorylation regulates the protein stability of KLF10 by interfering with binding of the E3 ligase SIAH1

Ching-Hui Lin, Shu-Yu Lin, Hsuen-Wen Chang, Li-Jung Ko, Yan-Shen Tseng, Vincent H S Chang, Winston C Y Yu

研究成果: 雜誌貢獻文章

2 引文 斯高帕斯(Scopus)

摘要

Downregulation of multiple cell cycle-regulatory molecules is a dominant event in TGF-β1-mediated growth inhibition of human carcinoma cells. It is known that KLF10 mimics the anti-proliferative and apoptotic effects that TGF-β1 has on epithelial cell growth and the growth of various tumor cells; based on these findings it is considered as a tumor suppressor. KLF10 protein expression is tightly associated with cell cycle-dependent events. However, the regulatory mechanism and its biological meaning have not been identified. In this study, we have demonstrated that KLF10 is a substrate of CDK2/cyclin E and can be phosphorylated. We also have shown that KLF10 efficiently binds to CDK2, while binding much less to CDK4, and displaying no binding to Cdk6. Using mass spectrometry, site direct mutagenesis, in vitro kinase assays and depletion assays, we have established that CDK2 phosphorylates Ser206, which subsequently affects the steady state level of KLF10 in cells. Our studies have also proved that CDK2 up-regulates the protein level of KLF10 through reducing its association with SIAH1, a KLF10 E3-ubiqutin ligase involved in proteasomal degradation. Taken all together, these findings indicate that CDK2-dependent phosphorylation regulates KLF10 stability and that this affects the role of KLF10 in cell.
原文英語
頁(從 - 到)1174-1181
頁數8
期刊Biochimica et Biophysica Acta - Molecular Cell Research
1853
發行號5
DOIs
出版狀態已發佈 - 五月 1 2015

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Medicine(all)

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