CD24 expression indicates healthier phenotype and less tendency of cellular senescence in human nucleus pulposus cells

Shu Hua Yang, Ming Hsiao Hu, Chang Chin Wu, Chih Wei Chen, Yuan Hui Sun, Kai Chiang Yang

研究成果: 雜誌貢獻文章

摘要

Identification of specific cell markers is crucial for recognizing functionally healthy nucleus pulposus (NP) cells. The objective of this study was to investigate the role of CD24 expression in adult human NP cells. Cells were retrieved from NP tissues of 20 patients (aged 17–44) operated on for lumbar disc herniation. Based on CD24 expression, NP cells were separated by sorting and then used to examine phenotypic behavior, the effects of culture conditions and cellular senescence pathway related proteins. CD24 expression was positive in 35.5 ± 3.7% (range 9.1–65.2%) of NP cells. Consistently, normoxic expansion and serial passages in monolayers decreased percentage positivity for CD24 in NP cells. CD24 NP cells showed a markedly decreased GSK-3β activity and increased mitogen-activated protein kinase phosphorylation accompanying by an increased β-catenin expression. Higher levels of matrix metalloproteinases, as well as lower levels of ACAN and COL2 in CD24 cells, indicated the breakdown and reduced the formation of key extracellular matrix components. CD24+ NP cells presented a more favorable phenotype while CD24 cells showed a more prominent cellular senescence fate. CD24 in NP cells may be a surrogate marker of healthy cells, in the cell-based therapeutic treatment of degenerative disc disorders.

原文英語
頁(從 - 到)3021-3028
頁數8
期刊Artificial Cells, Nanomedicine and Biotechnology
47
發行號1
DOIs
出版狀態已發佈 - 十二月 4 2019

ASJC Scopus subject areas

  • Biotechnology
  • Medicine (miscellaneous)
  • Biomedical Engineering
  • Pharmaceutical Science

指紋 深入研究「CD24 expression indicates healthier phenotype and less tendency of cellular senescence in human nucleus pulposus cells」主題。共同形成了獨特的指紋。

  • 引用此