Casticin induced apoptotic cell death and altered associated gene expression in human colon cancer colo 205 cells

Hung Sheng Shang, Jia You Liu, Hsu Feng Lu, Han Sun Chiang, Chia Hain Lin, Ann Chen, Yuh Feng Lin, Jing Gung Chung

研究成果: 雜誌貢獻文章

9 引文 斯高帕斯(Scopus)

摘要

Casticin, a polymethoxyflavone, derived from natural plant Fructus Viticis exhibits biological activities including anti-cancer characteristics. The anti-cancer and alter gene expression of casticin on human colon cancer cells and the underlying mechanisms were investigated. Flow cytometric assay was used to measure viable cell, cell cycle and sub-G1 phase, reactive oxygen species (ROS) and Ca2+ productions, level of mitochondria membrane potential (ΔΨm) and caspase activity. Western blotting assay was used to detect expression of protein level associated with cell death. Casticin induced cell morphological changes, decreased cell viability and induced G2/M phase arrest in colo 205 cells. Casticin increased ROS production but decreased the levels of ΔΨm, and Ca2+, increased caspase-3, -8, and -9 activities. The cDNA microarray indicated that some of the cell cycle associated genes were down-regulated such as cyclin-dependent kinase inhibitor 1A (CDKN1A) (p21, Cip1) and p21 protein (Cdc42/Rac)-activated kinase 3 (PAK3). TNF receptor-associated protein 1 (TRAP1), CREB1 (cAMP responsive element binding protein 1) and cyclin-dependent kinase inhibitor 1B (CDKN1B) (p27, Kip1) genes were increased but matrix metallopeptidase 2 (MMP-2), toll-like receptor 4 (TLR4), PRKAR2B (protein kinase, cAMP-dependent, regulatory, type II, bet), and CaMK4 (calcium/calmodulin-dependent protein kinase IV) genes were inhibited. Results suggest that casticin induced cell apoptosis via the activation of the caspase- and/or mitochondria-dependent signaling cascade, the accumulation of ROS and altered associated gene expressions in colo 205 human colon cancer cells.

原文英語
期刊Environmental Toxicology
DOIs
出版狀態接受/付印 - 2016

ASJC Scopus subject areas

  • Toxicology
  • Management, Monitoring, Policy and Law
  • Health, Toxicology and Mutagenesis

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    Shang, H. S., Liu, J. Y., Lu, H. F., Chiang, H. S., Lin, C. H., Chen, A., Lin, Y. F., & Chung, J. G. (認可的出版社/出版中). Casticin induced apoptotic cell death and altered associated gene expression in human colon cancer colo 205 cells. Environmental Toxicology. https://doi.org/10.1002/tox.22381