Cartilage regeneration in SCID mice using a highly organized three-dimensional alginate scaffold

Chen Chie Wang, Kai Chiang Yang, Keng Hui Lin, Yen Liang Liu, Hwa Chang Liu, Feng Huei Lin

研究成果: 雜誌貢獻文章

36 引文 (Scopus)

摘要

Tissue engineering for cartilage regeneration provides an alternative to surgery for degenerative osteoarthritis. Recently, a highly organized three-dimensional (3D) alginate scaffold was prepared using a microfluidic device; this scaffold is effective for chondrocyte culture in vitro. The performance of this scaffold was further demonstrated; an alginate scaffold seeded with porcine chondrocytes was implanted in the dorsal subcutaneous site of SCID mice. The recipients were sacrificed at 2, 4, and 6 weeks after transplantation. The grafted implants retrieved from the subcutaneous site were analyzed with histologic examinations. Real-time PCR was used to identify the gene expression patterns of the chondrocytes. The hematoxylin and eosin staining showed that the chondrocytes survived normally in SCID mice; cartilage-like structures were formed after 4 weeks implantation. Immunohistochemical staining revealed cells secreted type II collagen, produced glycosaminoglycans (proved by alcian blue stain), and maintained the expression of S-100. On the other hand, the cells were negative for type I and type X collagen staining. PCR showed that the mRNA expressions of aggrecan and type II collagen were up-regulated at weeks two and four, while type I and type X collagen were down-regulated during the study period. In summary, this highly organized 3D alginate scaffold provided a suitable environment and maintained functional phenotypes for chondrocytes in this animal study.
原文英語
頁(從 - 到)120-127
頁數8
期刊Biomaterials
33
發行號1
DOIs
出版狀態已發佈 - 一月 2012

指紋

SCID Mice
Alginate
Cartilage
Scaffolds (biology)
Chondrocytes
Scaffolds
Regeneration
Collagen
Collagen Type X
Collagen Type II
Lab-On-A-Chip Devices
Staining and Labeling
Collagen Type I
Aggrecans
Alcian Blue
Hematoxylin
Tissue Engineering
Eosine Yellowish-(YS)
Glycosaminoglycans
Tissue engineering

ASJC Scopus subject areas

  • Biomaterials
  • Bioengineering
  • Ceramics and Composites
  • Mechanics of Materials
  • Biophysics

引用此文

Cartilage regeneration in SCID mice using a highly organized three-dimensional alginate scaffold. / Wang, Chen Chie; Yang, Kai Chiang; Lin, Keng Hui; Liu, Yen Liang; Liu, Hwa Chang; Lin, Feng Huei.

於: Biomaterials, 卷 33, 編號 1, 01.2012, p. 120-127.

研究成果: 雜誌貢獻文章

Wang, Chen Chie ; Yang, Kai Chiang ; Lin, Keng Hui ; Liu, Yen Liang ; Liu, Hwa Chang ; Lin, Feng Huei. / Cartilage regeneration in SCID mice using a highly organized three-dimensional alginate scaffold. 於: Biomaterials. 2012 ; 卷 33, 編號 1. 頁 120-127.
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