Carp gamma-crystallins with high methionine content: Cloning and sequencing of the complementary DNA

Tschining Chang, Yun Jin Jiang, Shyh Horng Chiou, Wen Chang Chang

研究成果: 雜誌貢獻文章

29 引文 (Scopus)

摘要

The nucleotide sequences of γ-crystallin cDNAs cloned from the common carp (Cyprinus carpio) have been determined. The amino-acid sequences derived consist of two polypeptides with 177 and 172 amino-acid residues for γ-m1 and γ-m2, respectively. They exhibit unusually high methionine contents: 12.4% for γ-m1 and 14% for γ-m2. Comparison of both fish γ-crystallins with bovine γ-II crystallin reveals that they are similar in structure. The striking features of both fish γ-crystallins are as follows. (1) Both of them retain the 'conserved' residues, i.e., Tyr-6, Glu-7, Gly-13, Ser-34 and their equivalents in other motifs. (2) they possess the second aromatic residue at position 11. Both of these structural features are considered to be the major factors in stabilizing the folded hairpin structure of the protein. (3) The variable residues in the core region of C-terminal domain are almost all sulfur-containing amino acids, i.e., methionine or cysteine. (4) 30% of the surface hydrophobic groups are composed of methionine. The last two unusual features have been found so far only in these two fish γ-crystallins. The high methionine content may make an important contribution to the protein stability of fish γ-crystallins.
原文英語
頁(從 - 到)226-229
頁數4
期刊BBA - Gene Structure and Expression
951
發行號1
DOIs
出版狀態已發佈 - 十一月 10 1988
對外發佈Yes

指紋

gamma-Crystallins
Crystallins
Carps
Cloning
Methionine
Organism Cloning
Complementary DNA
Fish
Fishes
Amino Acids
Sulfur Amino Acids
Protein Stability
Sulfur
Cysteine
Amino Acid Sequence
Proteins
Nucleotides
Peptides

ASJC Scopus subject areas

  • Structural Biology
  • Biophysics
  • Biochemistry
  • Genetics

引用此文

Carp gamma-crystallins with high methionine content : Cloning and sequencing of the complementary DNA. / Chang, Tschining; Jiang, Yun Jin; Chiou, Shyh Horng; Chang, Wen Chang.

於: BBA - Gene Structure and Expression, 卷 951, 編號 1, 10.11.1988, p. 226-229.

研究成果: 雜誌貢獻文章

Chang, Tschining ; Jiang, Yun Jin ; Chiou, Shyh Horng ; Chang, Wen Chang. / Carp gamma-crystallins with high methionine content : Cloning and sequencing of the complementary DNA. 於: BBA - Gene Structure and Expression. 1988 ; 卷 951, 編號 1. 頁 226-229.
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abstract = "The nucleotide sequences of γ-crystallin cDNAs cloned from the common carp (Cyprinus carpio) have been determined. The amino-acid sequences derived consist of two polypeptides with 177 and 172 amino-acid residues for γ-m1 and γ-m2, respectively. They exhibit unusually high methionine contents: 12.4{\%} for γ-m1 and 14{\%} for γ-m2. Comparison of both fish γ-crystallins with bovine γ-II crystallin reveals that they are similar in structure. The striking features of both fish γ-crystallins are as follows. (1) Both of them retain the 'conserved' residues, i.e., Tyr-6, Glu-7, Gly-13, Ser-34 and their equivalents in other motifs. (2) they possess the second aromatic residue at position 11. Both of these structural features are considered to be the major factors in stabilizing the folded hairpin structure of the protein. (3) The variable residues in the core region of C-terminal domain are almost all sulfur-containing amino acids, i.e., methionine or cysteine. (4) 30{\%} of the surface hydrophobic groups are composed of methionine. The last two unusual features have been found so far only in these two fish γ-crystallins. The high methionine content may make an important contribution to the protein stability of fish γ-crystallins.",
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T2 - Cloning and sequencing of the complementary DNA

AU - Chang, Tschining

AU - Jiang, Yun Jin

AU - Chiou, Shyh Horng

AU - Chang, Wen Chang

PY - 1988/11/10

Y1 - 1988/11/10

N2 - The nucleotide sequences of γ-crystallin cDNAs cloned from the common carp (Cyprinus carpio) have been determined. The amino-acid sequences derived consist of two polypeptides with 177 and 172 amino-acid residues for γ-m1 and γ-m2, respectively. They exhibit unusually high methionine contents: 12.4% for γ-m1 and 14% for γ-m2. Comparison of both fish γ-crystallins with bovine γ-II crystallin reveals that they are similar in structure. The striking features of both fish γ-crystallins are as follows. (1) Both of them retain the 'conserved' residues, i.e., Tyr-6, Glu-7, Gly-13, Ser-34 and their equivalents in other motifs. (2) they possess the second aromatic residue at position 11. Both of these structural features are considered to be the major factors in stabilizing the folded hairpin structure of the protein. (3) The variable residues in the core region of C-terminal domain are almost all sulfur-containing amino acids, i.e., methionine or cysteine. (4) 30% of the surface hydrophobic groups are composed of methionine. The last two unusual features have been found so far only in these two fish γ-crystallins. The high methionine content may make an important contribution to the protein stability of fish γ-crystallins.

AB - The nucleotide sequences of γ-crystallin cDNAs cloned from the common carp (Cyprinus carpio) have been determined. The amino-acid sequences derived consist of two polypeptides with 177 and 172 amino-acid residues for γ-m1 and γ-m2, respectively. They exhibit unusually high methionine contents: 12.4% for γ-m1 and 14% for γ-m2. Comparison of both fish γ-crystallins with bovine γ-II crystallin reveals that they are similar in structure. The striking features of both fish γ-crystallins are as follows. (1) Both of them retain the 'conserved' residues, i.e., Tyr-6, Glu-7, Gly-13, Ser-34 and their equivalents in other motifs. (2) they possess the second aromatic residue at position 11. Both of these structural features are considered to be the major factors in stabilizing the folded hairpin structure of the protein. (3) The variable residues in the core region of C-terminal domain are almost all sulfur-containing amino acids, i.e., methionine or cysteine. (4) 30% of the surface hydrophobic groups are composed of methionine. The last two unusual features have been found so far only in these two fish γ-crystallins. The high methionine content may make an important contribution to the protein stability of fish γ-crystallins.

KW - (C. carpis)

KW - Amino acid sequence

KW - cDNA

KW - DNA sequence

KW - Methionine content

KW - Sequence homology

KW - γ-Crystallin

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