Rationale: CARD-recruited membrane-associated protein 3 (CARMA3) is a novel scaffold protein that regulates nuclear factor (NF)-κB activation; however, the underlying mechanism of CARMA3 in lung cancer stemness and metastasis remains largely unknown. Objectives: To investigate the molecular mechanisms underlying the involvement of CARMA3 in non-small cell lung cancer progression. Methods: The expression levels of CARMA3 and NME2 in a cohort of patients with lung cancer (n = 91) were examined by immunohistochemistry staining and assessed by Kaplan-Meier survival analysis. The effects of CARMA3, microRNA-182 (miR-182), and NME2 on cancer stemness and metastasis were measured in vitro and in vivo. Chromatin immunoprecipitation and luciferase reporter assays were performed to determine the mechanisms of NF-κB-driven miR-182 expression and NME2 regulation. Measurements and Main Results: We observed that CARMA3 inversely correlated with NME2 expression in patients with lung cancer (Pearson correlation coefficient: R = -0.24; P = 0.022). NME2 levels were significantly decreased in tumor tissues compared with adjacent normal lung tissues (P <0.001), and patients with lung cancer with higher levels of NME2 had longer survival outcomes (overall survival, P <0.01; disease-free survival, P <0.01). Mechanistically, CARMA3 promoted cell motility by reducing the level of NME2 through the NF-κB/miR-182 pathway and by increasing cancer stem cell properties and metastasis in lung cancer. Conclusions: We identified a novel mechanism of CARMA3 in lung cancer stemness and metastasis through the negative regulation of NME2 by NF-κB-dependent induction of miR-182. Our findings provide an attractive strategy for targeting the CARMA3/NF-κB/miR-182 pathway as a potential treatment for lung cancer.
|頁（從 - 到）||64-75|
|期刊||American Journal of Respiratory and Critical Care Medicine|
|出版狀態||已發佈 - 七月 1 2015|
ASJC Scopus subject areas
- Pulmonary and Respiratory Medicine
- Critical Care and Intensive Care Medicine
Chang, Y. W., Chiu, C. F., Lee, K. Y., Hong, C. C., Wang, Y. Y., Cheng, C. C., Jan, Y. H., Huang, M. S., Hsiao, M., Ma, J. T., & Su, J. L. (2015). CARMA3 represses metastasis suppressor NME2 to promote lung cancer stemness and metastasis. American Journal of Respiratory and Critical Care Medicine, 192(1), 64-75. https://doi.org/10.1164/rccm.201411-1957OC