Capsaicin may induce breast cancer cell death through apoptosis-inducing factor involving mitochondrial dysfunction

H. C. Chang, S. T. Chen, S. Y. Chien, S. J. Kuo, H. T. Tsai, D. R. Chen

研究成果: 雜誌貢獻文章

33 引文 (Scopus)

摘要

The majority of breast cancer patients are resistant to chemotherapy or radiotherapy due to the down-regulation or lack of caspase-3 expression. Capsaicin was found to inhibit cancer cell growth in caspase-3-deficient human breast cancer cells. This study aimed to investigate the growth-inhibitive effect of capsaicin and its mechanisms in human breast cancer cell lines, MCF-7 and BT-20. The results showed that cell viability decreased in a dose-dependent manner in both the caspase-3-deficient and non-deficient cells through inducing cell apoptosis and arresting the cell cycle in the S phase. Capsaicin significantly decreased mitochondria membrane potential, induced the cleavage of PARP-1, and decreased procaspase-7 expression in both cells. Apoptosis-inducing factor (AIF) was distinctly released from mitochondria and translocated into the cytoplasm and nucleus in MCF-7 cells (52.9%), but not in BT-20 cells (2%) after treatment with 200 μM of capsaicin for 24 hours. Capsaicin inhibited breast cancer cell growth through inducing cell apoptosis and cell cycle arrest in the S phase. This apoptotic effect could be induced through the mitochondrial pathway, and PARP-1 subsequently cleaved by activation of caspase-7. The application of capsaicin in clinical therapy could be useful for breast cancer patients.

原文英語
頁(從 - 到)1657-1665
頁數9
期刊Human and Experimental Toxicology
30
發行號10
DOIs
出版狀態已發佈 - 十月 2011
對外發佈Yes

指紋

Apoptosis Inducing Factor
Capsaicin
Cell death
Cell Death
Breast Neoplasms
Cells
Caspase 3
Caspase 7
Mitochondria
Cell growth
S Phase
Apoptosis
Chemotherapy
Growth
Radiotherapy
MCF-7 Cells
Cell Cycle Checkpoints
Chemical activation
Membrane Potentials
Membranes

ASJC Scopus subject areas

  • Toxicology
  • Health, Toxicology and Mutagenesis

引用此文

Capsaicin may induce breast cancer cell death through apoptosis-inducing factor involving mitochondrial dysfunction. / Chang, H. C.; Chen, S. T.; Chien, S. Y.; Kuo, S. J.; Tsai, H. T.; Chen, D. R.

於: Human and Experimental Toxicology, 卷 30, 編號 10, 10.2011, p. 1657-1665.

研究成果: 雜誌貢獻文章

Chang, H. C. ; Chen, S. T. ; Chien, S. Y. ; Kuo, S. J. ; Tsai, H. T. ; Chen, D. R. / Capsaicin may induce breast cancer cell death through apoptosis-inducing factor involving mitochondrial dysfunction. 於: Human and Experimental Toxicology. 2011 ; 卷 30, 編號 10. 頁 1657-1665.
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