Campylobacter jejuni Cytolethal Distending Toxin C Exploits Lipid Rafts to Mitigate Helicobacter pylori-Induced Pathogenesis

Jia Yin Yeh, Hwai Jeng Lin, Chia Jung Kuo, Chun Lung Feng, Chia Huei Chou, Chia Der Lin, Hui Yu Wu, Chen Yi Li, Cheng Hsun Chiu, Chih Ho Lai

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1 引文 斯高帕斯(Scopus)

摘要

Helicobacter pylori infection is associated with several gastrointestinal diseases, including gastritis, peptic ulcer, and gastrointestinal adenocarcinoma. Two major cytotoxins, vacuolating cytotoxin A (VacA) and cytotoxin-associated gene A (CagA), interact closely with lipid rafts, contributing to H. pylori-associated disease progression. The Campylobacter jejuni cytolethal distending toxin consists of three subunits: CdtA, CdtB, and CdtC. Among them, CdtA and CdtC bind to membrane lipid rafts, which is crucial for CdtB entry into cells. In this study, we employed recombinant CdtC (rCdtC) to antagonize the functions of H. pylori cytotoxin in cells. Our results showed that rCdtC alleviates cell vacuolation induced by H. pylori VacA. Furthermore, rCdtC reduces H. pylori CagA translocation, which decreases nuclear factor kappa-B activation and interleukin-8 production, resulting in the mitigation of gastric epithelial cell inflammation. These results reveal that CdtC hijacks cholesterol to compete for H. pylori cytotoxin actions via lipid rafts, ameliorating H. pylori-induced pathogenesis.

原文英語
文章編號617419
期刊Frontiers in Cell and Developmental Biology
8
DOIs
出版狀態已發佈 - 二月 23 2021

ASJC Scopus subject areas

  • 發展生物學
  • 細胞生物學

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