摘要

Ovarian granulose cells are known to play a key role in regulating ovarian physiology. Age increases apoptosis in follicular granulose cells and subsequently decreases ovarian fecundity. The aging ovary also contains fewer follicles that possess fewer granulose cells. The viability of follicular granulosa cells may be essential for development of the oocyte. Calcium ion plays an important role in a variety of biological processes, including gene expression, cell cycle regulation, and cell death. To study the ability of mitochondrial biogenesis in human granulosa cells, we determined the mitochondrial marker proteins, including the nuclear-encoded NADH-ubiquinone oxidoreductase alpha subunit 9 (NDUFA9) and mitochondrial-encoded COX I, after treatment of the cells with the calcium ionophore A23187. We showed that the expression of these mitochondrial marker proteins in human granulosa cells increased with changes in cytosolic Ca2+ using the ionophore A23187. Treatment of granulosa cells with 0.5 μM of A23187 for 120 h increased the levels of NDUFA 9 and COX I subunit by up to 2.6- and 2.4-fold, respectively. Raising Ca2+ by exposing granulosa cells to 1 μM of A23187 for 48 h significantly increased mitochondrial transcription factor (mtTFA) gene expression by up to 2.9-fold. Our results indicate that the adaptive responses of granulosa cells to increased Ca2+ may include upregulation of mitochondrial proteins and that mtTFA may be involved in such a mitochondrial biogenesis pathway.
原文英語
頁(從 - 到)157-162
頁數6
期刊Annals of the New York Academy of Sciences
1042
DOIs
出版狀態已發佈 - 2005

指紋

Granulosa Cells
Organelle Biogenesis
Calcimycin
Calcium
Gene expression
Mitochondrial Proteins
Transcription Factors
Cells
Electron Transport Complex I
Calcium Ionophores
Ionophores
Physiology
Cell death
Proteins
Aging of materials
Ions
Apoptosis
Biological Phenomena
Gene Expression
Oocytes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • History and Philosophy of Science

引用此文

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title = "Calcium stimulates mitochondrial biogenesis in human granulosa cells",
abstract = "Ovarian granulose cells are known to play a key role in regulating ovarian physiology. Age increases apoptosis in follicular granulose cells and subsequently decreases ovarian fecundity. The aging ovary also contains fewer follicles that possess fewer granulose cells. The viability of follicular granulosa cells may be essential for development of the oocyte. Calcium ion plays an important role in a variety of biological processes, including gene expression, cell cycle regulation, and cell death. To study the ability of mitochondrial biogenesis in human granulosa cells, we determined the mitochondrial marker proteins, including the nuclear-encoded NADH-ubiquinone oxidoreductase alpha subunit 9 (NDUFA9) and mitochondrial-encoded COX I, after treatment of the cells with the calcium ionophore A23187. We showed that the expression of these mitochondrial marker proteins in human granulosa cells increased with changes in cytosolic Ca2+ using the ionophore A23187. Treatment of granulosa cells with 0.5 μM of A23187 for 120 h increased the levels of NDUFA 9 and COX I subunit by up to 2.6- and 2.4-fold, respectively. Raising Ca2+ by exposing granulosa cells to 1 μM of A23187 for 48 h significantly increased mitochondrial transcription factor (mtTFA) gene expression by up to 2.9-fold. Our results indicate that the adaptive responses of granulosa cells to increased Ca2+ may include upregulation of mitochondrial proteins and that mtTFA may be involved in such a mitochondrial biogenesis pathway.",
keywords = "Calcium, Granulose cell, Mitochondria",
author = "Tian-Shun Tsai and Ho, {Jau Der} and Yang, {Vivian Wei Chung} and Tzeng, {Chii Ruey} and Hsieh, {Rong Hong}",
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pages = "157--162",
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T1 - Calcium stimulates mitochondrial biogenesis in human granulosa cells

AU - Tsai, Tian-Shun

AU - Ho, Jau Der

AU - Yang, Vivian Wei Chung

AU - Tzeng, Chii Ruey

AU - Hsieh, Rong Hong

PY - 2005

Y1 - 2005

N2 - Ovarian granulose cells are known to play a key role in regulating ovarian physiology. Age increases apoptosis in follicular granulose cells and subsequently decreases ovarian fecundity. The aging ovary also contains fewer follicles that possess fewer granulose cells. The viability of follicular granulosa cells may be essential for development of the oocyte. Calcium ion plays an important role in a variety of biological processes, including gene expression, cell cycle regulation, and cell death. To study the ability of mitochondrial biogenesis in human granulosa cells, we determined the mitochondrial marker proteins, including the nuclear-encoded NADH-ubiquinone oxidoreductase alpha subunit 9 (NDUFA9) and mitochondrial-encoded COX I, after treatment of the cells with the calcium ionophore A23187. We showed that the expression of these mitochondrial marker proteins in human granulosa cells increased with changes in cytosolic Ca2+ using the ionophore A23187. Treatment of granulosa cells with 0.5 μM of A23187 for 120 h increased the levels of NDUFA 9 and COX I subunit by up to 2.6- and 2.4-fold, respectively. Raising Ca2+ by exposing granulosa cells to 1 μM of A23187 for 48 h significantly increased mitochondrial transcription factor (mtTFA) gene expression by up to 2.9-fold. Our results indicate that the adaptive responses of granulosa cells to increased Ca2+ may include upregulation of mitochondrial proteins and that mtTFA may be involved in such a mitochondrial biogenesis pathway.

AB - Ovarian granulose cells are known to play a key role in regulating ovarian physiology. Age increases apoptosis in follicular granulose cells and subsequently decreases ovarian fecundity. The aging ovary also contains fewer follicles that possess fewer granulose cells. The viability of follicular granulosa cells may be essential for development of the oocyte. Calcium ion plays an important role in a variety of biological processes, including gene expression, cell cycle regulation, and cell death. To study the ability of mitochondrial biogenesis in human granulosa cells, we determined the mitochondrial marker proteins, including the nuclear-encoded NADH-ubiquinone oxidoreductase alpha subunit 9 (NDUFA9) and mitochondrial-encoded COX I, after treatment of the cells with the calcium ionophore A23187. We showed that the expression of these mitochondrial marker proteins in human granulosa cells increased with changes in cytosolic Ca2+ using the ionophore A23187. Treatment of granulosa cells with 0.5 μM of A23187 for 120 h increased the levels of NDUFA 9 and COX I subunit by up to 2.6- and 2.4-fold, respectively. Raising Ca2+ by exposing granulosa cells to 1 μM of A23187 for 48 h significantly increased mitochondrial transcription factor (mtTFA) gene expression by up to 2.9-fold. Our results indicate that the adaptive responses of granulosa cells to increased Ca2+ may include upregulation of mitochondrial proteins and that mtTFA may be involved in such a mitochondrial biogenesis pathway.

KW - Calcium

KW - Granulose cell

KW - Mitochondria

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