Objective: To evaluate the transcription and translation ability of mitochondria in terminally differentiated granulosa cells, these cells were incubated with ionic calcium. Design: Prospective laboratory research. Setting: In vitro fertilization laboratory in a university hospital. Patient(s): Granulosa cells were harvested from 50 female patients undergoing IVF. Intervention(s): Analysis of mitochondrial gene expression by semiquantitative reverse transcription-polymerase chain reaction (RT-PCR) and of mitochondrial-encoded proteins by Western blot. Main Outcome Measure(s): Comparison of the RNA expression levels of genes including cytochrome c oxidase subunit I (COX I), adenosine triphosphate synthase 6 (ATPase 6), flavoprotein, and succinate-ubiquinone oxidoreductase, and protein levels of COX I and flavoprotein in different calcium ion treatment groups. Result(s): There were dose-dependent increases in RNA expressions of the four genes analyzed from granulosa cells cultured in a serial concentration of calcium ions. This effect was abolished when cells were preincubated with the extracellular calcium-chelating agent, Ethylene glycol-bis (2-aminoethylether)-N,N,N′, N′-tetraacetic acid (EGTA). The effect of ionic calcium on both the nuclear- and mitochondrial-encoded subunits also was determined. Expression levels of mitochondrial transcription factor A in RNA significantly increased in granulosa cells that were exposed for 24 and 48 hours to 0.5 and 1 μM A23187. Conclusion(s): The present study is the first report to present calcium-dependent increases in the transcription and translation levels of both nuclear-encoded and mitochondrial-encoded mitochondrial respiratory enzyme subunits and also indicates that mitochondrial transcription factor A is involved in mitochondrial biogenesis.
ASJC Scopus subject areas
- Obstetrics and Gynaecology
Au, H-K., Yeh, T-S., Kao, S-H., Shih, C-M., Hsieh, R-H., & Tzeng, C-R. (2005). Calcium-dependent up-regulation of mitochondrial electron transfer chain gene expressions in human luteinized granulosa cells. Fertility and Sterility, 84(SUPPL. 2), 1104-1108. https://doi.org/10.1016/j.fertnstert.2005.03.072