摘要

Objective: To evaluate the transcription and translation ability of mitochondria in terminally differentiated granulosa cells, these cells were incubated with ionic calcium. Design: Prospective laboratory research. Setting: In vitro fertilization laboratory in a university hospital. Patient(s): Granulosa cells were harvested from 50 female patients undergoing IVF. Intervention(s): Analysis of mitochondrial gene expression by semiquantitative reverse transcription-polymerase chain reaction (RT-PCR) and of mitochondrial-encoded proteins by Western blot. Main Outcome Measure(s): Comparison of the RNA expression levels of genes including cytochrome c oxidase subunit I (COX I), adenosine triphosphate synthase 6 (ATPase 6), flavoprotein, and succinate-ubiquinone oxidoreductase, and protein levels of COX I and flavoprotein in different calcium ion treatment groups. Result(s): There were dose-dependent increases in RNA expressions of the four genes analyzed from granulosa cells cultured in a serial concentration of calcium ions. This effect was abolished when cells were preincubated with the extracellular calcium-chelating agent, Ethylene glycol-bis (2-aminoethylether)-N,N,N′, N′-tetraacetic acid (EGTA). The effect of ionic calcium on both the nuclear- and mitochondrial-encoded subunits also was determined. Expression levels of mitochondrial transcription factor A in RNA significantly increased in granulosa cells that were exposed for 24 and 48 hours to 0.5 and 1 μM A23187. Conclusion(s): The present study is the first report to present calcium-dependent increases in the transcription and translation levels of both nuclear-encoded and mitochondrial-encoded mitochondrial respiratory enzyme subunits and also indicates that mitochondrial transcription factor A is involved in mitochondrial biogenesis.
原文英語
頁(從 - 到)1104-1108
頁數5
期刊Fertility and Sterility
84
發行號SUPPL. 2
DOIs
出版狀態已發佈 - 十月 2005

指紋

Granulosa Cells
Up-Regulation
Electrons
Calcium
Gene Expression
Flavoproteins
RNA
Electron Transport Complex II
Ions
Mitochondrial Genes
Ethylene Glycol
Egtazic Acid
Mitochondrial Proteins
Calcimycin
Organelle Biogenesis
Electron Transport Complex IV
Fertilization in Vitro
Reverse Transcription
Mitochondria
Oxidoreductases

ASJC Scopus subject areas

  • Obstetrics and Gynaecology

引用此文

@article{f0f936eeff1948808adffdecd4ba3654,
title = "Calcium-dependent up-regulation of mitochondrial electron transfer chain gene expressions in human luteinized granulosa cells",
abstract = "Objective: To evaluate the transcription and translation ability of mitochondria in terminally differentiated granulosa cells, these cells were incubated with ionic calcium. Design: Prospective laboratory research. Setting: In vitro fertilization laboratory in a university hospital. Patient(s): Granulosa cells were harvested from 50 female patients undergoing IVF. Intervention(s): Analysis of mitochondrial gene expression by semiquantitative reverse transcription-polymerase chain reaction (RT-PCR) and of mitochondrial-encoded proteins by Western blot. Main Outcome Measure(s): Comparison of the RNA expression levels of genes including cytochrome c oxidase subunit I (COX I), adenosine triphosphate synthase 6 (ATPase 6), flavoprotein, and succinate-ubiquinone oxidoreductase, and protein levels of COX I and flavoprotein in different calcium ion treatment groups. Result(s): There were dose-dependent increases in RNA expressions of the four genes analyzed from granulosa cells cultured in a serial concentration of calcium ions. This effect was abolished when cells were preincubated with the extracellular calcium-chelating agent, Ethylene glycol-bis (2-aminoethylether)-N,N,N′, N′-tetraacetic acid (EGTA). The effect of ionic calcium on both the nuclear- and mitochondrial-encoded subunits also was determined. Expression levels of mitochondrial transcription factor A in RNA significantly increased in granulosa cells that were exposed for 24 and 48 hours to 0.5 and 1 μM A23187. Conclusion(s): The present study is the first report to present calcium-dependent increases in the transcription and translation levels of both nuclear-encoded and mitochondrial-encoded mitochondrial respiratory enzyme subunits and also indicates that mitochondrial transcription factor A is involved in mitochondrial biogenesis.",
keywords = "Granulosa cells, Mitochondria, RT-PCR",
author = "Heng-Kien Au and Tian-Shun Yeh and Shu-Huei Kao and Chwen-Ming Shih and Rong-Hong Hsieh and Chii-Ruey Tzeng",
year = "2005",
month = "10",
doi = "10.1016/j.fertnstert.2005.03.072",
language = "English",
volume = "84",
pages = "1104--1108",
journal = "Fertility and Sterility",
issn = "0015-0282",
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number = "SUPPL. 2",

}

TY - JOUR

T1 - Calcium-dependent up-regulation of mitochondrial electron transfer chain gene expressions in human luteinized granulosa cells

AU - Au, Heng-Kien

AU - Yeh, Tian-Shun

AU - Kao, Shu-Huei

AU - Shih, Chwen-Ming

AU - Hsieh, Rong-Hong

AU - Tzeng, Chii-Ruey

PY - 2005/10

Y1 - 2005/10

N2 - Objective: To evaluate the transcription and translation ability of mitochondria in terminally differentiated granulosa cells, these cells were incubated with ionic calcium. Design: Prospective laboratory research. Setting: In vitro fertilization laboratory in a university hospital. Patient(s): Granulosa cells were harvested from 50 female patients undergoing IVF. Intervention(s): Analysis of mitochondrial gene expression by semiquantitative reverse transcription-polymerase chain reaction (RT-PCR) and of mitochondrial-encoded proteins by Western blot. Main Outcome Measure(s): Comparison of the RNA expression levels of genes including cytochrome c oxidase subunit I (COX I), adenosine triphosphate synthase 6 (ATPase 6), flavoprotein, and succinate-ubiquinone oxidoreductase, and protein levels of COX I and flavoprotein in different calcium ion treatment groups. Result(s): There were dose-dependent increases in RNA expressions of the four genes analyzed from granulosa cells cultured in a serial concentration of calcium ions. This effect was abolished when cells were preincubated with the extracellular calcium-chelating agent, Ethylene glycol-bis (2-aminoethylether)-N,N,N′, N′-tetraacetic acid (EGTA). The effect of ionic calcium on both the nuclear- and mitochondrial-encoded subunits also was determined. Expression levels of mitochondrial transcription factor A in RNA significantly increased in granulosa cells that were exposed for 24 and 48 hours to 0.5 and 1 μM A23187. Conclusion(s): The present study is the first report to present calcium-dependent increases in the transcription and translation levels of both nuclear-encoded and mitochondrial-encoded mitochondrial respiratory enzyme subunits and also indicates that mitochondrial transcription factor A is involved in mitochondrial biogenesis.

AB - Objective: To evaluate the transcription and translation ability of mitochondria in terminally differentiated granulosa cells, these cells were incubated with ionic calcium. Design: Prospective laboratory research. Setting: In vitro fertilization laboratory in a university hospital. Patient(s): Granulosa cells were harvested from 50 female patients undergoing IVF. Intervention(s): Analysis of mitochondrial gene expression by semiquantitative reverse transcription-polymerase chain reaction (RT-PCR) and of mitochondrial-encoded proteins by Western blot. Main Outcome Measure(s): Comparison of the RNA expression levels of genes including cytochrome c oxidase subunit I (COX I), adenosine triphosphate synthase 6 (ATPase 6), flavoprotein, and succinate-ubiquinone oxidoreductase, and protein levels of COX I and flavoprotein in different calcium ion treatment groups. Result(s): There were dose-dependent increases in RNA expressions of the four genes analyzed from granulosa cells cultured in a serial concentration of calcium ions. This effect was abolished when cells were preincubated with the extracellular calcium-chelating agent, Ethylene glycol-bis (2-aminoethylether)-N,N,N′, N′-tetraacetic acid (EGTA). The effect of ionic calcium on both the nuclear- and mitochondrial-encoded subunits also was determined. Expression levels of mitochondrial transcription factor A in RNA significantly increased in granulosa cells that were exposed for 24 and 48 hours to 0.5 and 1 μM A23187. Conclusion(s): The present study is the first report to present calcium-dependent increases in the transcription and translation levels of both nuclear-encoded and mitochondrial-encoded mitochondrial respiratory enzyme subunits and also indicates that mitochondrial transcription factor A is involved in mitochondrial biogenesis.

KW - Granulosa cells

KW - Mitochondria

KW - RT-PCR

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U2 - 10.1016/j.fertnstert.2005.03.072

DO - 10.1016/j.fertnstert.2005.03.072

M3 - Article

C2 - 16210000

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VL - 84

SP - 1104

EP - 1108

JO - Fertility and Sterility

JF - Fertility and Sterility

SN - 0015-0282

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ER -