CACC box and enhancer response of the human embryonic ε globin promoter

Kouros Motamed, Carol Bastiani, Zhang Qingyi, Arnold Bailey, C. K.James Shen

研究成果: 雜誌貢獻文章同行評審

8 引文 斯高帕斯(Scopus)

摘要

The functional interaction between the human ε globin promoter and an erythroid-specific transcription enhancer, 5′ HS-2, has been analyzed by transient expression assay. While stepwise deletion of DNA sequences between -852 and -122 had only small effects, removal of the CACC box at position -111 greatly decreased ε-globin promoter activity, as well as its response to the enhancer function of 5′ HS-2 in erythroid cells. Our data demonstrated that the three ubiquitous promoter elements, the CACC, CCAAT, and TATA boxes, of the ε-globin-encoding gene together form a minimal promoter that would interact efficiently with 5′ HS-2, and that at least the CACC box is an essential functional component of this enhancer-promoter interaction.

原文英語
頁(從 - 到)235-240
頁數6
期刊Gene
123
發行號2
DOIs
出版狀態已發佈 - 一月 30 1993
對外發佈

ASJC Scopus subject areas

  • 遺傳學

指紋

深入研究「CACC box and enhancer response of the human embryonic ε globin promoter」主題。共同形成了獨特的指紋。

引用此