C-terminus of Hsc70-interacting protein (CHIP) enhances stemness properties of human Wharton’s jelly mesenchymal stem cell

C. K. Yang, C. C. Feng, J. F. Lo, J. W. Chen, V. V. Padma, C. H. Lai, T. S. Chen, R. J. Chen, P. H. Liao, C. Y. Huang

研究成果: 雜誌貢獻文章

摘要

Mesenchymal stem cells are an attractive source of multipotent cells in part because they are easy to obtain. Several E3 ligases regulate the stability and functions of various factors in different adult stem cells through the ubiquitylation pathway. We investigated the C-terminus of Hsc70-interacting protein (CHIP) E3 ligase that regulates pluripotency of human Wharton’s jelly mesenchymal stem cells (hWJMSC). We found that CHIP increases protein kinase B (Akt) phosphorylation by decreased expression of phosphatase and tensin homolog (PTEN), which suggests improvement of the survival pathway by CHIP over-expression. We also found that increased CHIP expression induced Sox2 and NANOG, which can promote stem cell self-renewal and prevent oxidative stress-induced senescence of hWJMSC by decreased p21. We found that CHIP could be used to enhance the multiple functions of hWJMSC.
原文英語
頁(從 - 到)632-639
頁數8
期刊Biotechnic and Histochemistry
93
發行號8
DOIs
出版狀態已發佈 - 十一月 17 2018

指紋

Wharton Jelly
HSC70 Heat-Shock Proteins
Mesenchymal Stromal Cells
Ubiquitin-Protein Ligases
Proto-Oncogene Proteins c-akt
Adult Stem Cells
Ubiquitination
Phosphoric Monoester Hydrolases
Oxidative Stress
Phosphorylation
Survival

ASJC Scopus subject areas

  • Histology
  • Medical Laboratory Technology

引用此文

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title = "C-terminus of Hsc70-interacting protein (CHIP) enhances stemness properties of human Wharton’s jelly mesenchymal stem cell",
abstract = "Mesenchymal stem cells are an attractive source of multipotent cells in part because they are easy to obtain. Several E3 ligases regulate the stability and functions of various factors in different adult stem cells through the ubiquitylation pathway. We investigated the C-terminus of Hsc70-interacting protein (CHIP) E3 ligase that regulates pluripotency of human Wharton’s jelly mesenchymal stem cells (hWJMSC). We found that CHIP increases protein kinase B (Akt) phosphorylation by decreased expression of phosphatase and tensin homolog (PTEN), which suggests improvement of the survival pathway by CHIP over-expression. We also found that increased CHIP expression induced Sox2 and NANOG, which can promote stem cell self-renewal and prevent oxidative stress-induced senescence of hWJMSC by decreased p21. We found that CHIP could be used to enhance the multiple functions of hWJMSC.",
keywords = "Hsc70-interacting protein, human, mesenchymal stem cell, phosphatase and tensin homolog (PTEN), protein kinase B (Akt), self-renewal, Wharton’s jelly",
author = "Yang, {C. K.} and Feng, {C. C.} and Lo, {J. F.} and Chen, {J. W.} and Padma, {V. V.} and Lai, {C. H.} and Chen, {T. S.} and Chen, {R. J.} and Liao, {P. H.} and Huang, {C. Y.}",
year = "2018",
month = "11",
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AU - Yang, C. K.

AU - Feng, C. C.

AU - Lo, J. F.

AU - Chen, J. W.

AU - Padma, V. V.

AU - Lai, C. H.

AU - Chen, T. S.

AU - Chen, R. J.

AU - Liao, P. H.

AU - Huang, C. Y.

PY - 2018/11/17

Y1 - 2018/11/17

N2 - Mesenchymal stem cells are an attractive source of multipotent cells in part because they are easy to obtain. Several E3 ligases regulate the stability and functions of various factors in different adult stem cells through the ubiquitylation pathway. We investigated the C-terminus of Hsc70-interacting protein (CHIP) E3 ligase that regulates pluripotency of human Wharton’s jelly mesenchymal stem cells (hWJMSC). We found that CHIP increases protein kinase B (Akt) phosphorylation by decreased expression of phosphatase and tensin homolog (PTEN), which suggests improvement of the survival pathway by CHIP over-expression. We also found that increased CHIP expression induced Sox2 and NANOG, which can promote stem cell self-renewal and prevent oxidative stress-induced senescence of hWJMSC by decreased p21. We found that CHIP could be used to enhance the multiple functions of hWJMSC.

AB - Mesenchymal stem cells are an attractive source of multipotent cells in part because they are easy to obtain. Several E3 ligases regulate the stability and functions of various factors in different adult stem cells through the ubiquitylation pathway. We investigated the C-terminus of Hsc70-interacting protein (CHIP) E3 ligase that regulates pluripotency of human Wharton’s jelly mesenchymal stem cells (hWJMSC). We found that CHIP increases protein kinase B (Akt) phosphorylation by decreased expression of phosphatase and tensin homolog (PTEN), which suggests improvement of the survival pathway by CHIP over-expression. We also found that increased CHIP expression induced Sox2 and NANOG, which can promote stem cell self-renewal and prevent oxidative stress-induced senescence of hWJMSC by decreased p21. We found that CHIP could be used to enhance the multiple functions of hWJMSC.

KW - Hsc70-interacting protein

KW - human

KW - mesenchymal stem cell

KW - phosphatase and tensin homolog (PTEN)

KW - protein kinase B (Akt)

KW - self-renewal

KW - Wharton’s jelly

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