Branched i antigen regulated cell susceptibility against natural killer cytotoxicity through its N-linked glycosylation and overall expression

Yu Xuan Wu, Hsu Feng Lu, Yen Hsi Lin, Hui Yu Chuang, Shih Chi Su, Yi Jen Liao, Yuh Ching Twu

研究成果: 雜誌貢獻文章同行評審

摘要

Cell surface glycosylation has been known as an important modification process that can be targeted and manipulated by malignant cells to escape from host immunosurveillance. We previously showed that the blood group branched I antigen on the leukemia cell surface can regulate the cell susceptibility against natural killer (NK) cell-mediated cytotoxicity through interfering target-NK interaction. In this work, we first identified N-linkage as the major glycosylation linkage type for branched I glycan formation on leukemia cells, and this linkage was responsible for cell sensitivity against therapeutic NK-92MI targeting. Secondly, by examining different leukemia cell surface death receptors, we showed death receptor Fas had highest expressions in both Raji and TF-1a cells. Mutations on two Fas extracellular N-linkage sites (118 and 136) for glycosylation impaired activation of Fas-mediated apoptosis during NK-92MI cytotoxicity. Last, we found that the surface I antigen expression levels enable leukemia cells to respond differently against NK-92MI targeting. In low I antigen expressing K-562 cell, reduction of I antigen presence greatly reduced leukemia cell susceptibility against NK-92MI targeting. But in other high I antigen expressing leukemia cells, similar reduction in I antigen expression did not affect cell susceptibility.

原文英語
頁(從 - 到)624-635
頁數12
期刊Glycobiology
31
發行號5
DOIs
出版狀態已發佈 - 五月 1 2021

ASJC Scopus subject areas

  • 生物化學

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