Both dioscorin, the tuber storage protein of yam (Dioscorea alata cv. Tainong No. 1), and its peptic hydrolysates exhibited angiotensin converting enzyme inhibitory activities

Feng-Lin Hsu, Yaw Huei Lin, Mei Hsien Lee, Chuen Liang Lin, Wen Chi Hou

研究成果: 雜誌貢獻文章同行評審

93 引文 斯高帕斯(Scopus)

摘要

Dioscorin, the tuber storage protein of yam (Dioscorea alata cv. Tainong No. 1), was purified to homogeneity by DE-52 ion-exchange chromatography. This purified dioscorin was shown by spectrophotometric methods to inhibit angiotensin converting enzyme (ACE) in a dose-dependent manner (12.5-750 μg, respectively, 20.83-62.5% inhibitions) using N-[3-(2-furyl)acryloyl]-Phe-Gly-Gly (FAPGG) as substrates. The 50% inhibition (IC50) of ACE activity was 6.404 μM dioscorin (250 μg corresponding to 7.81 nmol) compared to that of 0.00781 μM (0.0095 nmol) for captopril. The commercial bovine serum albumin and casein (bovine milk) showed less ACE inhibitory activity. The use of qualitative TLC also showed dioscorin as ACE inhibitors. Dioscorin showed mixed noncompetitive inhibitions against ACE; when 31.25 μg of dioscorin (0.8 μM) was added, the apparent inhibition constant (Ki) was 2.738 μM. Pepsin was used for dioscorin hydrolysis at 37 °C for different times. It was found that the ACE inhibitory activity was increased from 51.32% to about 75% during 32 h hydrolysis. The smaller peptides were increased with increasing pepsin hydrolytic times. Dioscorin and its hydrolysates might be a potential for hypertension control when people consume yam tuber.

原文英語
頁(從 - 到)6109-6113
頁數5
期刊Journal of Agricultural and Food Chemistry
50
發行號21
DOIs
出版狀態已發佈 - 10月 9 2002

ASJC Scopus subject areas

  • 農業與生物科學(雜項)
  • 食品科學
  • 化學(雜項)

指紋

深入研究「Both dioscorin, the tuber storage protein of yam (Dioscorea alata cv. Tainong No. 1), and its peptic hydrolysates exhibited angiotensin converting enzyme inhibitory activities」主題。共同形成了獨特的指紋。

引用此