Biomolecular clusters distribution up to mega dalton region using MALDI-quadrupole ion trap mass spectrometer

Yung Kun Chuang, Szu Hsueh Lai, Jung Lee Lin, Chung Hsuan Chen

研究成果: 雜誌貢獻文章

1 引文 (Scopus)

摘要

We present the first report on complete cluster distributions of cytochrome c (molecular weight of 12.4 kDa) and bovine serum albumin ((BSA), molecular weight of 66.4 kDa) with mass-to-charge ratio (m/z) reaching 350,000 and 1,400,000, respectively, by matrix-assisted laser desorption/ionization (MALDI). Large cluster distributions of the analytes were measured by our homemade frequency-scanned quadrupole ion trap (QIT) mass spectrometer with a charge detector. To our knowledge, we report the highest m/z clusters of these two biomolecules. The quantitative results indicate that large clusters ions of cytochrome c and BSA follow the power law (r2 > 0.99) with cluster size distribution, which provides experimental evidence for the laser ablation studies of MALDI.
原文英語
文章編號2789
期刊International Journal of Molecular Sciences
19
發行號9
DOIs
出版狀態已發佈 - 九月 17 2018

指紋

Mass spectrometers
Bovine Serum Albumin
Cytochromes c
mass spectrometers
Ionization
Desorption
Lasers
quadrupoles
Molecular Weight
desorption
Molecular weight
Ions
Proteins
ionization
Laser Therapy
Biomolecules
Laser ablation
matrices
cytochromes
lasers

ASJC Scopus subject areas

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

引用此文

Biomolecular clusters distribution up to mega dalton region using MALDI-quadrupole ion trap mass spectrometer. / Chuang, Yung Kun; Lai, Szu Hsueh; Lin, Jung Lee; Chen, Chung Hsuan.

於: International Journal of Molecular Sciences, 卷 19, 編號 9, 2789, 17.09.2018.

研究成果: 雜誌貢獻文章

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AU - Chuang, Yung Kun

AU - Lai, Szu Hsueh

AU - Lin, Jung Lee

AU - Chen, Chung Hsuan

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N2 - We present the first report on complete cluster distributions of cytochrome c (molecular weight of 12.4 kDa) and bovine serum albumin ((BSA), molecular weight of 66.4 kDa) with mass-to-charge ratio (m/z) reaching 350,000 and 1,400,000, respectively, by matrix-assisted laser desorption/ionization (MALDI). Large cluster distributions of the analytes were measured by our homemade frequency-scanned quadrupole ion trap (QIT) mass spectrometer with a charge detector. To our knowledge, we report the highest m/z clusters of these two biomolecules. The quantitative results indicate that large clusters ions of cytochrome c and BSA follow the power law (r2 > 0.99) with cluster size distribution, which provides experimental evidence for the laser ablation studies of MALDI.

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