Biofilm formation is not associated with worse outcome in Acinetobacter baumannii bacteraemic pneumonia

Yung Chih Wang, Tzu Wen Huang, Ya Sung Yang, Shu Chen Kuo, Chung Ting Chen, Chang Pan Liu, Yuag Meng Liu, Te Li Chen, Feng Yee Chang, Shih Hsiung Wu, Chorng Kuang How, Yi Tzu Lee

研究成果: 雜誌貢獻文章

9 引文 斯高帕斯(Scopus)

摘要

The effect of biofilm formation on bacteraemic pneumonia caused by A. baumannii is unknown. We conducted a 4-year multi-center retrospective study to analyze 71 and 202 patients with A. baumannii bacteraemic pneumonia caused by biofilm-forming and non-biofilm-forming isolates, respectively. The clinical features and outcomes of patients were investigated. Biofilm formation was determined by a microtitre plate assay. The antimicrobial susceptibilities of biofilm-associated cells were assessed using the minimum biofilm eradication concentration (MBEC) assay. Whole-genome sequencing was conducted to identify biofilm-associated genes and their promoters. Quantitative reverse transcription polymerase chain reaction was performed to confirm the expression difference of biofilm-associated genes. There was no significant difference in the clinical characteristics or the outcomes between patients infected with biofilm-forming and non-biofilm-forming strains. Compared with non-biofilm-forming isolates, biofilm-forming isolates exhibited lower resistance to most antimicrobials tested, including imipenem, meropenem, ceftazidime, ciprofloxacin and gentamicin; however, the MBEC assay confirmed the increased antibiotic resistance of the biofilm-embedded bacteria. Biofilm-associated genes and their promoters were detected in most isolates, including the non-biofilm-forming strains. Biofilm-forming isolates showed higher levels of expression of the biofilm-associated genes than non-biofilm-forming isolates. The biofilm-forming ability of A. baumannii isolates might not be associated with worse outcomes in patients with bacteraemic pneumonia.
原文英語
文章編號7289
期刊Scientific Reports
8
發行號1
DOIs
出版狀態已發佈 - 十二月 1 2018

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    Wang, Y. C., Huang, T. W., Yang, Y. S., Kuo, S. C., Chen, C. T., Liu, C. P., Liu, Y. M., Chen, T. L., Chang, F. Y., Wu, S. H., How, C. K., & Lee, Y. T. (2018). Biofilm formation is not associated with worse outcome in Acinetobacter baumannii bacteraemic pneumonia. Scientific Reports, 8(1), [7289]. https://doi.org/10.1038/s41598-018-25661-9