摘要

Mg-based alloys have great potential for development into fixation implants because of their highly biocompatible and biodegradable metallic properties. In this study, we sought to determine the biocompatibility of Mg 60 Zn 35 Ca 5 bulk metallic glass composite (BMGC) with fabricated implants in a rabbit tendon-bone interference fixation model. We investigated the cellular cytotoxicity of Mg 60 Zn 35 Ca 5 BMGC toward rabbit osteoblasts and compared it with conventional titanium alloy (Ti6Al4V) and polylactic acid (PLA). The results show that Mg 60 Zn 35 Ca 5 BMGC may be classed as slightly toxic on the basis of the standard ISO 10993-5. We further characterized the osteogenic effect of the Mg 60 Zn 35 Ca 5 BMGC extraction medium on rabbit osteoblasts by quantifying extracellular calcium and mineral deposition, as well as cellular alkaline phosphatase activity. The results of these tests were found to be promising. The chemotactic effect of the Mg 60 Zn 35 Ca 5 BMGC extraction medium on rabbit osteoblasts was demonstrated through a transwell migration assay. For the in vivo section of this study, a rabbit tendon-bone interference fixation model was established to determine the biocompatibility and osteogenic potential of Mg 60 Zn 35 Ca 5 BMGC in a created bony tunnel for a period of up to 24 weeks. The results show that Mg 60 Zn 35 Ca 5 BMGC induced considerable new bone formation at the implant site in comparison with conventional titanium alloy after 24 weeks of implantation. In conclusion, this study revealed that Mg 60 Zn 35 Ca 5 BMGC demonstrated adequate biocompatibility and exhibited significant osteogenic potential both in vitro and in vivo. These advantages may be clinically beneficial to the development of Mg 60 Zn 35 Ca 5 BMGC implants for future applications.
原文英語
文章編號2191
期刊International Journal of Molecular Sciences
20
發行號9
DOIs
出版狀態已發佈 - 五月 3 2019

指紋

tendons
Tendons
rabbits
Metallic glass
biocompatibility
metallic glasses
Biocompatibility
bones
Glass
Bone
Rabbits
interference
Bone and Bones
composite materials
Composite materials
osteoblasts
Osteoblasts
titanium alloys
Titanium
Titanium alloys

ASJC Scopus subject areas

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

引用此文

Biocompatibility and osteogenic capacity of Mg-Zn-Ca Bulk metallic glass for rabbit tendon-bone interference fixation. / Wong, Chin Chean; Wong, Pei Chun; Tsai, Pei Hua; Jang, Jason Shian Ching; Cheng, Cheng Kung; Chen, Hsiang Ho; Chen, Chih Hwa.

於: International Journal of Molecular Sciences, 卷 20, 編號 9, 2191, 03.05.2019.

研究成果: 雜誌貢獻文章

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title = "Biocompatibility and osteogenic capacity of Mg-Zn-Ca Bulk metallic glass for rabbit tendon-bone interference fixation",
abstract = "Mg-based alloys have great potential for development into fixation implants because of their highly biocompatible and biodegradable metallic properties. In this study, we sought to determine the biocompatibility of Mg 60 Zn 35 Ca 5 bulk metallic glass composite (BMGC) with fabricated implants in a rabbit tendon-bone interference fixation model. We investigated the cellular cytotoxicity of Mg 60 Zn 35 Ca 5 BMGC toward rabbit osteoblasts and compared it with conventional titanium alloy (Ti6Al4V) and polylactic acid (PLA). The results show that Mg 60 Zn 35 Ca 5 BMGC may be classed as slightly toxic on the basis of the standard ISO 10993-5. We further characterized the osteogenic effect of the Mg 60 Zn 35 Ca 5 BMGC extraction medium on rabbit osteoblasts by quantifying extracellular calcium and mineral deposition, as well as cellular alkaline phosphatase activity. The results of these tests were found to be promising. The chemotactic effect of the Mg 60 Zn 35 Ca 5 BMGC extraction medium on rabbit osteoblasts was demonstrated through a transwell migration assay. For the in vivo section of this study, a rabbit tendon-bone interference fixation model was established to determine the biocompatibility and osteogenic potential of Mg 60 Zn 35 Ca 5 BMGC in a created bony tunnel for a period of up to 24 weeks. The results show that Mg 60 Zn 35 Ca 5 BMGC induced considerable new bone formation at the implant site in comparison with conventional titanium alloy after 24 weeks of implantation. In conclusion, this study revealed that Mg 60 Zn 35 Ca 5 BMGC demonstrated adequate biocompatibility and exhibited significant osteogenic potential both in vitro and in vivo. These advantages may be clinically beneficial to the development of Mg 60 Zn 35 Ca 5 BMGC implants for future applications.",
keywords = "Biocompatible, Biodegradable, MgZnCa bulk metallic glass, Osteogenic",
author = "Wong, {Chin Chean} and Wong, {Pei Chun} and Tsai, {Pei Hua} and Jang, {Jason Shian Ching} and Cheng, {Cheng Kung} and Chen, {Hsiang Ho} and Chen, {Chih Hwa}",
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AU - Wong, Chin Chean

AU - Wong, Pei Chun

AU - Tsai, Pei Hua

AU - Jang, Jason Shian Ching

AU - Cheng, Cheng Kung

AU - Chen, Hsiang Ho

AU - Chen, Chih Hwa

PY - 2019/5/3

Y1 - 2019/5/3

N2 - Mg-based alloys have great potential for development into fixation implants because of their highly biocompatible and biodegradable metallic properties. In this study, we sought to determine the biocompatibility of Mg 60 Zn 35 Ca 5 bulk metallic glass composite (BMGC) with fabricated implants in a rabbit tendon-bone interference fixation model. We investigated the cellular cytotoxicity of Mg 60 Zn 35 Ca 5 BMGC toward rabbit osteoblasts and compared it with conventional titanium alloy (Ti6Al4V) and polylactic acid (PLA). The results show that Mg 60 Zn 35 Ca 5 BMGC may be classed as slightly toxic on the basis of the standard ISO 10993-5. We further characterized the osteogenic effect of the Mg 60 Zn 35 Ca 5 BMGC extraction medium on rabbit osteoblasts by quantifying extracellular calcium and mineral deposition, as well as cellular alkaline phosphatase activity. The results of these tests were found to be promising. The chemotactic effect of the Mg 60 Zn 35 Ca 5 BMGC extraction medium on rabbit osteoblasts was demonstrated through a transwell migration assay. For the in vivo section of this study, a rabbit tendon-bone interference fixation model was established to determine the biocompatibility and osteogenic potential of Mg 60 Zn 35 Ca 5 BMGC in a created bony tunnel for a period of up to 24 weeks. The results show that Mg 60 Zn 35 Ca 5 BMGC induced considerable new bone formation at the implant site in comparison with conventional titanium alloy after 24 weeks of implantation. In conclusion, this study revealed that Mg 60 Zn 35 Ca 5 BMGC demonstrated adequate biocompatibility and exhibited significant osteogenic potential both in vitro and in vivo. These advantages may be clinically beneficial to the development of Mg 60 Zn 35 Ca 5 BMGC implants for future applications.

AB - Mg-based alloys have great potential for development into fixation implants because of their highly biocompatible and biodegradable metallic properties. In this study, we sought to determine the biocompatibility of Mg 60 Zn 35 Ca 5 bulk metallic glass composite (BMGC) with fabricated implants in a rabbit tendon-bone interference fixation model. We investigated the cellular cytotoxicity of Mg 60 Zn 35 Ca 5 BMGC toward rabbit osteoblasts and compared it with conventional titanium alloy (Ti6Al4V) and polylactic acid (PLA). The results show that Mg 60 Zn 35 Ca 5 BMGC may be classed as slightly toxic on the basis of the standard ISO 10993-5. We further characterized the osteogenic effect of the Mg 60 Zn 35 Ca 5 BMGC extraction medium on rabbit osteoblasts by quantifying extracellular calcium and mineral deposition, as well as cellular alkaline phosphatase activity. The results of these tests were found to be promising. The chemotactic effect of the Mg 60 Zn 35 Ca 5 BMGC extraction medium on rabbit osteoblasts was demonstrated through a transwell migration assay. For the in vivo section of this study, a rabbit tendon-bone interference fixation model was established to determine the biocompatibility and osteogenic potential of Mg 60 Zn 35 Ca 5 BMGC in a created bony tunnel for a period of up to 24 weeks. The results show that Mg 60 Zn 35 Ca 5 BMGC induced considerable new bone formation at the implant site in comparison with conventional titanium alloy after 24 weeks of implantation. In conclusion, this study revealed that Mg 60 Zn 35 Ca 5 BMGC demonstrated adequate biocompatibility and exhibited significant osteogenic potential both in vitro and in vivo. These advantages may be clinically beneficial to the development of Mg 60 Zn 35 Ca 5 BMGC implants for future applications.

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KW - Biodegradable

KW - MgZnCa bulk metallic glass

KW - Osteogenic

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