Biochemical and physical properties of a solvent-detergent-treated fibrin glue

M. Burnouf-Radosevich, T. Burnouf, J. J. Huart

研究成果: 雜誌貢獻文章

42 引文 (Scopus)

摘要

A fibrin glue preparation has been obtained from pooled human plasma using a procedure which includes a solvent-detergent (SD) treatment to inactivate lipid-enveloped viruses. The SD treatment inactivated ≥5.5 log10 of HIV in less than 45 min, and ≥5 log10 and ≥6.5 log10 of VSV and Sindbis virus, respectively, in less than 2 h. The product was found to contain high quantities of fibrinogen (116±2.49 g/l; n = 12), factor XIII (35±2.88 U/ml) and von Willebrand factor (23±1.9 U/ml ristocetin cofactor activity), and relatively low levels of fibronectin (5.9±0.a51 g/l). Plasminogen, the precursor of plasmin, which may play a negativer role by decreasing the resistance of the fibrin clot, was at only 0.03 g/l. Cellulose acetate electrophoresis showed 95% gamma-proteins and 5% alpha-2-beta proteins. Sodium dodecyl sulfate polyacrylamide gel electrophoresis under reducing conditions detected three main protein bands with apparent molecular weights of 65, 56 and 47 kilodaltons, probably corresponding to the alpha, beta, and gamma fibrinogen subunits. Other characteristics of the product included (1) high clottability of fibrinogen (over 85%); (2) absence of low molecular weight fibrin degradation products; (3) rapid solubilization at room temperature (less than 10 min); (4) high tensile strength (202±27 g/cm2 after 2 h of application), and (5) high elasticity of the fibrin clot. In addition, scanning electron microscopy revealed a highly organized structure showing tridimensional arrangement of the fibrin fibers. SD treated fibrin glue should efficiently replace autologous fibrinogen or cryoprecipitate preparations for surgical application.
原文英語
頁(從 - 到)77-84
頁數8
期刊Vox Sanguinis
58
發行號2
出版狀態已發佈 - 1990
對外發佈Yes

指紋

Fibrin Tissue Adhesive
Detergents
Fibrinogen
Fibrin
von Willebrand Factor
Molecular Weight
Cellulose Acetate Electrophoresis
Sindbis Virus
Factor XIII
Fibrin Fibrinogen Degradation Products
Proteins
Tensile Strength
Plasminogen
Fibrinolysin
Elasticity
Fibronectins
Sodium Dodecyl Sulfate
Electron Scanning Microscopy
Polyacrylamide Gel Electrophoresis
HIV

ASJC Scopus subject areas

  • Hematology

引用此文

Biochemical and physical properties of a solvent-detergent-treated fibrin glue. / Burnouf-Radosevich, M.; Burnouf, T.; Huart, J. J.

於: Vox Sanguinis, 卷 58, 編號 2, 1990, p. 77-84.

研究成果: 雜誌貢獻文章

Burnouf-Radosevich, M, Burnouf, T & Huart, JJ 1990, 'Biochemical and physical properties of a solvent-detergent-treated fibrin glue', Vox Sanguinis, 卷 58, 編號 2, 頁 77-84.
Burnouf-Radosevich, M. ; Burnouf, T. ; Huart, J. J. / Biochemical and physical properties of a solvent-detergent-treated fibrin glue. 於: Vox Sanguinis. 1990 ; 卷 58, 編號 2. 頁 77-84.
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N2 - A fibrin glue preparation has been obtained from pooled human plasma using a procedure which includes a solvent-detergent (SD) treatment to inactivate lipid-enveloped viruses. The SD treatment inactivated ≥5.5 log10 of HIV in less than 45 min, and ≥5 log10 and ≥6.5 log10 of VSV and Sindbis virus, respectively, in less than 2 h. The product was found to contain high quantities of fibrinogen (116±2.49 g/l; n = 12), factor XIII (35±2.88 U/ml) and von Willebrand factor (23±1.9 U/ml ristocetin cofactor activity), and relatively low levels of fibronectin (5.9±0.a51 g/l). Plasminogen, the precursor of plasmin, which may play a negativer role by decreasing the resistance of the fibrin clot, was at only 0.03 g/l. Cellulose acetate electrophoresis showed 95% gamma-proteins and 5% alpha-2-beta proteins. Sodium dodecyl sulfate polyacrylamide gel electrophoresis under reducing conditions detected three main protein bands with apparent molecular weights of 65, 56 and 47 kilodaltons, probably corresponding to the alpha, beta, and gamma fibrinogen subunits. Other characteristics of the product included (1) high clottability of fibrinogen (over 85%); (2) absence of low molecular weight fibrin degradation products; (3) rapid solubilization at room temperature (less than 10 min); (4) high tensile strength (202±27 g/cm2 after 2 h of application), and (5) high elasticity of the fibrin clot. In addition, scanning electron microscopy revealed a highly organized structure showing tridimensional arrangement of the fibrin fibers. SD treated fibrin glue should efficiently replace autologous fibrinogen or cryoprecipitate preparations for surgical application.

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