Biochemical and biological properties of an α1-antitrypsin concentrate

T. Burnouf, J. Constans, A. Clerc, J. Descamps, L. Martinache, M. Goudemand

研究成果: 雜誌貢獻文章同行評審

32 引文 斯高帕斯(Scopus)

摘要

α1-Antitrypsin (AAT) has been purified from human plasma supernatant A (equivalent to COHN fraction II + III) by a large-scale chromatographic procedure involving anion-exchange adsorption on DEAE Sepharose CL-6B fast flow and size-exclusion chromatography on Sephacryl S-200. Before freeze-drying, the liquid concentrate was heat-treated at 60°C for 10 h to reduce the risk of transmission of blood-borne viral diseases. Using this procedure, AAT is recovered with 80-90% purity in 65-75% yield from supernatant A. The heterogeneity of AAT is preserved across the purification steps. In addition, purified AAT exhibits inhibitory activities against trypsin and elastase equivalent to that of the serum protein. The mean association rate constant for elastase was found as high as 2.15 x 105 M-1 s-1. Thus, purifying active AAT from supernatant A contributes to improving the availability of this protein which may be potentially useful in the treatment of hereditary emphysema.
原文英語
頁(從 - 到)291-297
頁數7
期刊Vox Sanguinis
52
發行號4
出版狀態已發佈 - 1987
對外發佈Yes

ASJC Scopus subject areas

  • Hematology

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